F
Fei Ran
Researcher at Massachusetts Institute of Technology
Publications - 26
Citations - 3800
Fei Ran is an academic researcher from Massachusetts Institute of Technology. The author has contributed to research in topics: CRISPR & Cas9. The author has an hindex of 10, co-authored 26 publications receiving 3718 citations.
Papers
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Crystal Structure of Cas9 in Complex with Guide RNA and Target DNA
Hiroshi Nishimasu,Naoshi Dohmae,Ryuichiro Ishitani,Osamu Nureki,Fei Ran,Patrick D. Hsu,Silvana Konermann,Soraya I. Shehata,Feng Zhang +8 more
TL;DR: The crystal structure of Streptococcus pyogenes Cas9 in complex with sgRNA and its target DNA at 2.5 Å resolution and accompanying functional analyses have revealed the molecular mechanism of RNA-guided DNA targeting by Cas9, paving the way for the rational design of new, versatile genome-editing technologies.
In vivo gene editing in dystrophic mouse muscle and muscle stem cells
Mohammadsharif Tabebordbar,Kexian Zhu,Jason Cheng,Wei Leong Chew,Jeffrey J. Widrick,Claire Maesner,Elizabeth Y Wu,Ru Xiao,Fei Ran,Luk H. Vandenberghe,George M. Church,Amy J. Wagers,Winston X. Yan,Le Cong,Feng Zhang +14 more
TL;DR: A direct gene-editing approach is developed and tested to induce exon deletion and recover dystrophin expression in the mdx mouse model of DMD and partially recovered muscle functional deficiencies and generated a pool of endogenously corrected myogenic precursors in m dx mouse muscle.
Patent
CRISPR-Cas component systems, methods and compositions for sequence manipulation
Feng Zhang,David Bikard,Le Cong,David Benjamin Turitz Cox,Patrick D. Hsu,Wenyan Jiang,Shauiliang Lin,Luciano A. Marraffini,Randall Jeffrey Platt,Fei Ran,Neville E. Sanjana +10 more
TL;DR: In this paper, the authors provide a system, methods, and compositions for manipulation of sequences and/or activities of target sequences, including vectors and vector systems, some of which encode one or more components of a CRISPR complex, and methods for the design and use of such vectors.
Patent
CRISPR-Cas Nickase Systems, Methods And Compositions For Sequence Manipulation in Eukaryotes
Feng Zhang,Le Cong,Fei Ran +2 more
TL;DR: In this paper, the authors provide a system, methods, and compositions for manipulation of sequences and/or activities of target sequences, including vectors and vector systems, some of which encode one or more components of a CRISPR complex, and methods for the design and use of such vectors.
Patent
Engineering of systems, methods and optimized guide compositions for sequence manipulation
TL;DR: The CRISPR-Cas system as discussed by the authors provides a set of vectors and vector systems, some of which encode one or more components of a CRisPR complex, as well as methods for the design and use of such vectors.