D
David McMillan
Researcher at UCB
Publications - 23
Citations - 778
David McMillan is an academic researcher from UCB. The author has contributed to research in topics: Receptor & Epitope. The author has an hindex of 11, co-authored 21 publications receiving 552 citations.
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Journal ArticleDOI
Biochemical characterization of the active heterodimer form of human heparanase (Hpa1) protein expressed in insect cells.
Edward A. McKenzie,Kathryn Young,Margaret L. Hircock,James Bennett,Maina Bhaman,Robert Felix,Paul Turner,Alasdair Stamps,David McMillan,Giles P Saville,Stanley Ng,Sean Mason,Daniel C. Snell,Darren Schofield,Haiping Gong,Reid Townsend,John T. Gallagher,Martin John Page,Raj Parekh,Colin Stubberfield +19 more
TL;DR: An insect cell expression system used for the generation of large amounts of recombinant protein of high specific activity is presented here for the first time and it is confirmed that the 8 kDa subunit is essential for enzyme activity.
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Structural basis for recognition of synaptic vesicle protein 2C by botulinum neurotoxin A
Roger Benoit,Daniel Frey,Manuel Hilbert,Josta T. Kevenaar,Mara M. Wieser,Christian U. Stirnimann,David McMillan,Tom Ceska,Florence Lebon,Rolf Jaussi,Michel O. Steinmetz,Gebhard F. X. Schertler,Casper C. Hoogenraad,Guido Capitani,Richard A. Kammerer +14 more
TL;DR: The high-resolution crystal structure of the BoNT/A receptor-binding domain (BoNT/ a-RBD) in complex with the SV2C luminal domain (SV2C-LD) is determined, providing a strong platform for the development of novel antitoxin agents and for the rational design of Bo NT/A variants with improved therapeutic properties.
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Epitope determines efficacy of therapeutic anti-Tau antibodies in a functional assay with human Alzheimer Tau
Jean-Philippe Courade,Rachel Angers,Georges Mairet-Coello,Nathalie Pacico,Kerry Louise Tyson,Daniel John Lightwood,Rebecca Munro,David McMillan,Robert G. Griffin,Terry Baker,Dale Starkie,Ruodan Nan,Marta Westwood,Marie-Laetitia Mushikiwabo,Sophie Jung,Geofrey Odede,Berni Sweeney,Andrew George Popplewell,Gillian Burgess,Patrick Downey,Martin Citron +20 more
TL;DR: A robust and quantitative cell based assay was developed and an unbiased screening approach was employed to identify the antibody with the highest activity against human Tau seeds, which potently blocked the seeding of human AD Tau and was fully efficacious against seeds from progressive supranuclear palsy.
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Characterization of the Interaction of Sclerostin with the Low Density Lipoprotein Receptor-related Protein (LRP) Family of Wnt Co-receptors *
Gill Holdsworth,Patrick M. Slocombe,Carl Brendan Doyle,Bernadette Sweeney,Vaclav Veverka,Kelly Le Riche,Richard J. Franklin,Joanne E. Compson,Daniel Christopher Brookings,James Petrie Turner,Jeffery Kennedy,Rachael Garlish,Jiye Shi,Laura Ellen Newnham,David McMillan,Mariusz Muzylak,Mark D. Carr,Alistair James Henry,Thomas A Ceska,Martyn K. Robinson +19 more
TL;DR: The crystal structure of the amino-terminal region of LRP6 is reported and using NMR show that the ability of sclerostin to bind to this molecule is mediated by the central core of s clerostin and does not involveThe amino- and carboxyl- terminal flexible arm regions.
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Small molecules that inhibit TNF signalling by stabilising an asymmetric form of the trimer.
O'connell James Philip,John Robert Porter,Boris Kroeplien,Timothy John Norman,Stephen Edward Rapecki,Rachel Davis,David McMillan,Tracy Arakaki,Alex B. Burgin,David A. Fox,Tom Ceska,Fabien Claude Lecomte,Alison Maloney,Alex Vugler,Bruce Carrington,Benjamin P. Cossins,Tim Bourne,Alastair D. G. Lawson +17 more
TL;DR: This discovery paves the way for a class of small molecule drugs capable of modulating TNF function by stabilising a naturally sampled, receptor-incompetent conformation of TNF, and may prove to be a more general mechanism for inhibiting protein–protein interactions.