Roger Benoit

TL;DR: The high-resolution crystal structure of the BoNT/A receptor-binding domain (BoNT/ a-RBD) in complex with the SV2C luminal domain (SV2C-LD) is determined, providing a strong platform for the development of novel antitoxin agents and for the rational design of Bo NT/A variants with improved therapeutic properties.

TL;DR: Crystallographic ‘snapshots’ taken at intervals of femtoseconds to milliseconds after activation show how a light-activated sodium pump carries sodium ions across the cell membrane and provide direct molecular insight into the dynamics of active cation transport across biological membranes.

TL;DR: An improved, universal method for the seamless integration of DNA fragments into plasmids at any desired position by Linearization of plasmid by polymerase chain reaction (PCR), instead of restriction enzyme digestion is described.

TL;DR: The data indicate that in vitro, HuR RRM1 andRRM1,2 homodimerization involves a disulfide bond at cysteine 13, which may have a functional significance in redox modulation of HuR activity in response to oxidative stress.

TL;DR: The identification of the first RRM3 (RNA recognition motif 3) targeted low molecular weight HuR inhibitors from a one-bead-one-compound library screen using confocal nanoscanning is described to suggest that HuR actively contributes to RNA modification and maturation and shed an entirely new light on the role of HuR in RNA metabolism.