Dimitra Aggeli

TL;DR: The high microalgal lipid potential is anticipated to force research towards finding effective ways to manipulate biochemical pathways involved in lipid biosynthesis and towards cost effective algal cultivation and harvesting systems, as well.

TL;DR: The most important efforts target the efficiency of the oleaginous machinery, via overexpression of key-enzymes involved in lipid biosynthesis, as well as the minimization of lipid degradation, by repressing genes involved in the β-oxidation pathway.

TL;DR: Oleaginous microorganisms are able to convert numerous agro-industrial and municipal wastes into storage lipids (single cell oil) and are therefore considered as potential biofuel producers and efforts should be focused in the construction of strains with high carbon uptake rates and a reprogrammed coordination of the individual parts of the oleganous machinery that maximizes carbon flux towards lipogenesis.

TL;DR: It is found that disruptive mutations, such as nonsense and frameshift, were much more common in the first step of adaptation, contributing an additional way that both diminishing returns and historical contingency are evident during 2nd step adaptation.

TL;DR: In this article, the authors study the extent of diminishing returns and the changes in the adaptive mutational spectra following a single first adaptive step and show that adaptive loss-of-function mutations such as nonsense and frameshift mutations are less common in the second step of adaptation than in the first step.