D
Dirk Heckl
Researcher at Martin Luther University of Halle-Wittenberg
Publications - 70
Citations - 8764
Dirk Heckl is an academic researcher from Martin Luther University of Halle-Wittenberg. The author has contributed to research in topics: Myeloid leukemia & Haematopoiesis. The author has an hindex of 22, co-authored 58 publications receiving 6883 citations. Previous affiliations of Dirk Heckl include Brigham and Women's Hospital & Hannover Medical School.
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Journal ArticleDOI
Genome-Scale CRISPR-Cas9 Knockout Screening in Human Cells
Ophir Shalem,Ophir Shalem,Neville E. Sanjana,Neville E. Sanjana,Ella Hartenian,Xi-Shun Shi,David A. Scott,David A. Scott,Tarjei S. Mikkelsen,Dirk Heckl,Benjamin L. Ebert,David E. Root,John G. Doench,Feng Zhang,Feng Zhang +14 more
TL;DR: This work shows that lentiviral delivery of a genome-scale CRISPR-Cas9 knockout (GeCKO) library targeting 18,080 genes with 64,751 unique guide sequences enables both negative and positive selection screening in human cells, and observes a high level of consistency between independent guide RNAs targeting the same gene and a high rate of hit confirmation.
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Lenalidomide Causes Selective Degradation of IKZF1 and IKZF3 in Multiple Myeloma Cells
Jan Krönke,Namrata D. Udeshi,Anupama Narla,Peter V. Grauman,Slater N. Hurst,Marie McConkey,Tanya Svinkina,Dirk Heckl,Eamon Comer,Xiaoyu Li,Christie Ciarlo,Emily C. Hartman,Nikhil C. Munshi,Monica Schenone,Stuart L. Schreiber,Steven A. Carr,Benjamin L. Ebert,Benjamin L. Ebert +17 more
TL;DR: Using quantitative proteomics, it is found that lenalidomide causes selective ubiquitination and degradation of two lymphoid transcription factors, IKZF1 and IKzF3, by the CRBN-CRL4 ubiquitin ligase, which are essential transcription factors in multiple myeloma.
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Single-cell RNA-seq reveals changes in cell cycle and differentiation programs upon aging of hematopoietic stem cells
Monika S. Kowalczyk,Itay Tirosh,Dirk Heckl,Tata Nageswara Rao,Tata Nageswara Rao,Atray Dixit,Brian J. Haas,Rebekka K. Schneider,Amy J. Wagers,Benjamin L. Ebert,Aviv Regev,Aviv Regev +11 more
TL;DR: It is found that cell cycle dominates the variability within each population and that there is a lower frequency of cells in the G1 phase among old compared with young long-term HSCs, suggesting that they traverse through G1 faster.
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Generation of mouse models of myeloid malignancy with combinatorial genetic lesions using CRISPR-Cas9 genome editing
Dirk Heckl,Monika S. Kowalczyk,David Yudovich,Roger Belizaire,Rishi V. Puram,Marie McConkey,Anne Thielke,Jon C. Aster,Aviv Regev,Benjamin L. Ebert +9 more
TL;DR: In this article, a lentivirus-delivered sgRNA:Cas9 genome editing was used to generate mice with acute myeloid leukemia (AML) with cooperating mutations in genes encoding epigenetic modifiers, transcription factors and mediators of cytokine signaling.
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Gli1+ Mesenchymal Stromal Cells Are a Key Driver of Bone Marrow Fibrosis and an Important Cellular Therapeutic Target
Rebekka K. Schneider,Rebekka K. Schneider,Ann Mullally,Aurelien Dugourd,Fabian Peisker,Remco Hoogenboezem,Paulina M. H. van Strien,Eric M.J. Bindels,Dirk Heckl,Guntram Büsche,David Fleck,Gerhard Müller-Newen,Janewit Wongboonsin,Janewit Wongboonsin,Mónica S. Ventura Ferreira,Victor G. Puelles,Julio Saez-Rodriguez,Benjamin L. Ebert,Benjamin D. Humphreys,Rafael Kramann +19 more
TL;DR: It is shown that Gli1+ mesenchymal stromal cells are recruited from the endosteal and perivascular niche to become fibrosis-driving myofibroblasts in the bone marrow, and Gli1 expression in BMF significantly correlates with the severity of the disease.