Ellie Ehrenfeld

TL;DR: It is shown how RNA viruses can manipulate multiple components of the cellular secretory pathway to generate organelles specialized for replication that are distinct in protein and lipid composition from the host cell.

TL;DR: Despite the membrane reorganization and vesicle formation induced by 2C and 2BC proteins, no enhanced synthesis of lipid was observed and this may indicate a novel property of 2C that induces a complex interaction with intracellular membranes.

TL;DR: The data show that PCBP2 is an essential factor, which is required for efficient translation of poliovirus RNA in HeLa cells.

TL;DR: Dicistronic RNAs containing mutations in the 5' cloverleaf-like structure of poliov virus that abate PCBP2 binding show a decrease in RNA replication and translation of gene products directed by the poliovirus 5' noncoding region in vitro, suggesting that the interaction of PCBP1 with these sequences performs a dual role in the virus life cycle by facilitating both viral protein synthesis and initiation of viral RNA synthesis.

TL;DR: The data show that the remodeling of cellular membranes in poliovirus-infected cells produces structures with positive curvature of membranes, suggesting there is a fundamental divergence in the requirements for the supporting cellular membrane-shaping machinery among different groups of positive-strand RNA viruses.