Erik Borgström

TL;DR: The method utilizes selective precipitation of certain sizes of DNA molecules on to paramagnetic beads for cleanup and selection after standard enzymatic reactions to generate libraries for de novo and re-sequencing on the Illumina HiSeq 2000 instrument.

TL;DR: Exome and whole-genome sequencing of single adipocytes suggests that BM/PBSC-derived progenitors contribute to adipose tissue via both differentiation and cell fusion, and at least in the setting of transplantation, BM serves as a reservoir for adipocyte progenitor cells, particularly in obese subjects.

TL;DR: Conclusively, the products from the AMPLI1 and MALBAC kits were shown to be most similar to the bulk samples and are therefore recommended for WGA of single cells.

TL;DR: The method enables use of widely available short-read-sequencing platforms to study long single molecules within a complex sample, without losing phase information.

TL;DR: Conbase leverages phased read data from multiple samples in a dataset to achieve increased confidence in somatic variant calls and genotype predictions and provides superior robustness on simulated data, in vitro expanded fibroblasts and clonal lymphocyte populations isolated directly from a healthy human donor.