Felicia Bratti

TL;DR: This work shows that metal-catalyzed autoxidation depolymerizes comingled polymers into a mixture of oxygenated small molecules that are advantaged substrates for biological conversion, and engineer a robust soil bacterium to funnel these oxygenated compounds into a single exemplary chemical product.

TL;DR: In this paper, the authors performed four sequential metabolic engineering efforts in Pseudomonas putida KT2440 to enable the conversion of PET glycolysis products via: (i) ethylene glycol utilization by constitutive expression of native genes, (ii) terephthalate (TPA) catabolism by expression of tphA2IIA3IIBIIA1II from Comamonas and tpaK from Rhodococcus jostii, (iii) bis(2-hydroxyethyl) TPA hydrolysis to TPA

TL;DR: This work obtained ADP1-derived strains capable of growing on TPA as the sole carbon source using chromosomal insertion and targeted amplification of the tph catabolic operon from Comamonas sp.

TL;DR: In this article, the performance of the wild-type and variant enzymes was compared as a function of temperature, substrate morphology, and reaction mixture composition, and it was shown that reaction temperature had the strongest influence on performance between the two enzymes.

TL;DR: In this article , the authors identify thermotolerant PET hydrolases from natural diversity by using bioinformatics and machine learning to mine 74 putative thermokinetic PET hydrogrolases.