Tandem chemical deconstruction and biological upcycling of poly(ethylene terephthalate) to β-ketoadipic acid by Pseudomonas putida KT2440.
Allison Z. Werner,Rita Clare,Mand Thomas David,Isabel Pardo,Kelsey J. Ramirez,Stefan J. Haugen,Felicia Bratti,Gara N. Dexter,Joshua R. Elmore,Jay D. Huenemann,George L. Peabody,Christopher W. Johnson,Nicholas A. Rorrer,Davinia Salvachúa,Adam M. Guss,Gregg T. Beckham +15 more
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TLDR
In this paper, the authors performed four sequential metabolic engineering efforts in Pseudomonas putida KT2440 to enable the conversion of PET glycolysis products via: (i) ethylene glycol utilization by constitutive expression of native genes, (ii) terephthalate (TPA) catabolism by expression of tphA2IIA3IIBIIA1II from Comamonas and tpaK from Rhodococcus jostii, (iii) bis(2-hydroxyethyl) TPA hydrolysis to TPAAbout:
This article is published in Metabolic Engineering.The article was published on 2021-07-12 and is currently open access. It has received 48 citations till now. The article focuses on the topics: Terephthalic acid & Ethylene glycol.read more
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Mechanism-Based Design of Efficient PET Hydrolases
Ren Wei,Gerlis von Haugwitz,Lara Anne Pfaff,Jan Mičan,Christoffel P. S. Badenhorst,Weidong Liu,Gert Weber,Harry P. Austin,David Bednar,Jiri Damborsky,Uwe T. Bornscheuer +10 more
TL;DR: Analysis of the specific material properties of PET and the reaction mechanisms in the context of interfacial biocatalysis identifies several limitations in current enzymatic PET degradation approaches that should be addressed in the near future.
Journal ArticleDOI
Mixed plastics waste valorization through tandem chemical oxidation and biological funneling
Kevin P. Sullivan,Allison Z. Werner,Kelsey J. Ramirez,Lucas D. Ellis,Jeremy R. Bussard,Brenna A. Black,David G. Brandner,Felicia Bratti,Bonnie L. Buss,Xueming Dong,Stefan J. Haugen,Morgan A Ingraham,Mikhail O. Konev,William E. Michener,Joel Miscall,Isabel Pardo,Sean P. Woodworth,Adam M. Guss,Yuriy Román-Leshkov,Shannon S. Stahl,Gregg T. Beckham +20 more
TL;DR: This work shows that metal-catalyzed autoxidation depolymerizes comingled polymers into a mixture of oxygenated small molecules that are advantaged substrates for biological conversion, and engineer a robust soil bacterium to funnel these oxygenated compounds into a single exemplary chemical product.
Journal ArticleDOI
Chemoautotroph Cupriavidus necator as a potential game-changer for global warming and plastic waste problem: A review.
Yu Jung Sohn,Jina Son,Seo Young Jo,Se Young Park,Jee In Yoo,Kei-Anne Baritugo,Jeong-Geol Na,Jong-il Choi,Hee Taek Kim,Jeong Chan Joo,Si Jae Park +10 more
TL;DR: Cupriavidus necator has been extensively examined for producing Polyhydroxyalkanoates (PHAs), the promising polyester alternatives to petroleum-based synthetic polymers because it has a superior ability for accumulating a considerable amount of PHAs from renewable resources.
Journal ArticleDOI
Plastic Waste Valorization by Leveraging Multidisciplinary Catalytic Technologies
Journal ArticleDOI
Particle Size Reduction of Poly(ethylene terephthalate) Increases the Rate of Enzymatic Depolymerization But Does Not Increase the Overall Conversion Extent
Richard K. Brizendine,Erika Erickson,Stefan J. Haugen,Kelsey J. Ramirez,Joel Miscall,Davinia Salvachúa,Andrew R. Pickford,Margaret J. Sobkowicz,John McGeehan,Gregg T. Beckham +9 more
TL;DR: In this article , the effect of particle size and crystallinity on the performance of a variant of the leaf compost-cutinase enzyme (LCC-ICCG) was investigated.
References
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Journal ArticleDOI
A Bacterium That Degrades and Assimilates Poly(ethylene Terephthalate)
Shosuke Yoshida,Kazumi Hiraga,Toshihiko Takehana,Ikuo Taniguchi,Hironao Yamaji,Yasuhito Maeda,Kiyotsuna Toyohara,Kenji Miyamoto,Yoshiharu Kimura,Kohei Oda +9 more
TL;DR: In this paper, a new bacterium, Ideonella sakaiensis 201-F6, was found to be able to use PET as its major energy and carbon source, producing two enzymes capable of hydrolyzing PET and the reaction intermediate, mono(2-hydroxyethyl) terephthalic acid.
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