Frederik Dethloff

TL;DR: This book chapter describes the analytical procedures required for the profiling of a metabolite fraction enriched for primary metabolites based on routine gas chromatography coupled to mass spectrometry (GC-MS), which is adapted to plant material that was exposed to temperature stress.

TL;DR: Untargeted mass spectrometry and 16S rRNA profiling of fecal pellet extracts showed several primary and secondary bile acid level, and microbiota alpha diversity differences, respectively between paroxetine- and vehicle-treated mice, suggesting that microbiota functions are altered by the drug.

TL;DR: The presented feeding assays and data evaluation strategies represent a rapid and easy-to-use tool box for enhanced metabolomics studies that combine differential pool size analysis with screening for differential carbon utilization from defined stable isotope labeled metabolic precursors.

TL;DR: It is demonstrated that REIL proteins interact in yeast-2-hybrid assays with A. thaliana homologs of the yeast proteins and take part in the cytosolic 60S ribosomal maturation process within yeast and physically interact with Rei1.

TL;DR: Partially 13C-labeled mouse plasma resulted in accurate relative quantification of human plasma amino acids using liquid chromatography coupled to mass spectrometry, and the coefficient of variation for the relative quantisation is reduced from 27% without internal standard to 10% with inclusion of partially 13C -labeled internal standard.