G
G. N. De Iuliis
Researcher at University of Newcastle
Publications - 18
Citations - 1763
G. N. De Iuliis is an academic researcher from University of Newcastle. The author has contributed to research in topics: DNA damage & Oxidative stress. The author has an hindex of 15, co-authored 18 publications receiving 1546 citations. Previous affiliations of G. N. De Iuliis include Newcastle University & IT University.
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On the possible origins of DNA damage in human spermatozoa
TL;DR: A two-step hypothesis for the origins of DNA damage in human spermatozoa is presented that highlights the significance of oxidative stress acting on vulnerable, poorly protaminated cells generated as a result of defective spermiogenesis.
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Cryopreservation-induced human sperm DNA damage is predominantly mediated by oxidative stress rather than apoptosis
Laura Kelly Thomson,Steven D. Fleming,Robert John Aitken,G. N. De Iuliis,Julie-Anne Zieschang,Anne Melton Clark +5 more
TL;DR: Human sperm DNA fragmentation is associated with an increase in oxidative stress during cryopreservation, rather than the activation of caspases and apoptosis, and the estrogenic compound genistein may be useful in reducing this effect.
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The TUNEL assay consistently underestimates DNA damage in human spermatozoa and is influenced by DNA compaction and cell vitality: development of an improved methodology.
TL;DR: The TUNEL methodology was further refined to incorporate a vital stain that covalently bound to intracellular amine groups in non-viable cells in order to facilitate research into the causes of DNA damage in human spermatozoa.
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The source and significance of DNA damage in human spermatozoa; a commentary on diagnostic strategies and straw man fallacies
TL;DR: The origins of DNA damage in human spermatozoa, the methods that are available to monitor this aspect of semen quality and the clinical significance of such measurements are discussed in this paper.
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On methods for the detection of reactive oxygen species generation by human spermatozoa: analysis of the cellular responses to catechol oestrogen, lipid aldehyde, menadione and arachidonic acid.
Robert John Aitken,T. B. Smith,Tessa Lord,L. Kuczera,A. J. Koppers,Nenad Naumovski,Haley S. Connaughton,Mark Baker,G. N. De Iuliis +8 more
TL;DR: Examination of spontaneous ROS generation by defective human spermatozoa revealed that MitoSOX Red was the most effective indicator of oxidative stress, thereby emphasizing the general importance of mitochondrial dysregulation in the aetiology of defective sperm function.