Gerald T. Marsischky

TL;DR: A proteomics approach to enrich, recover, and identify ubiquitin conjugates from Saccharomyces cerevisiae lysate provides a general tool for the large-scale analysis and characterization of protein ubiquitination.

TL;DR: Elevated expression of Rab1, the mammalian YPT1 homolog, protected against αSyn-induced dopaminergic neuron loss in animal models of PD, suggesting synucleinopathies may result from disruptions in basic cellular functions that interface with the unique biology of particular neurons to make them especially vulnerable.

TL;DR: Eukaryotic mismatch repair (MMR) has been shown to require two different heterodimeric complexes of MutS-related proteins: MSH 2-MSH3 and MSH2- MSH6, and alternative models have been proposed for how these MSH complexes function in MMR.

TL;DR: The results indicate that S. cerevisiae has two pathways of MSH 2-dependent mismatch repair: one that recognized single-base mispairs and requires MSH2 and MSH6, and a second that recognizes insertion/deletion mispaired and requires a combination of either MSH1-6 or MSh2 andMSH6.

TL;DR: Analysis of the mismatched nucleotide-binding specificity of the hMSH2-hMSH3 and hMSh2-HMSH6 protein complexes showed that they have overlapping but not identical binding specificity, which helps to explain the distribution of mutations in different mismatch-repair genes seen in hereditary nonpolyposis colon cancer.