Jeroen Roelofs

TL;DR: In this paper, the authors present a set of guidelines for the selection and interpretation of methods for use by investigators who aim to examine macro-autophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes.

TL;DR: A proteomics approach to enrich, recover, and identify ubiquitin conjugates from Saccharomyces cerevisiae lysate provides a general tool for the large-scale analysis and characterization of protein ubiquitination.

TL;DR: In this article, the authors present a set of guidelines for investigators to select and interpret methods to examine autophagy and related processes, and for reviewers to provide realistic and reasonable critiques of reports that are focused on these processes.

TL;DR: The recent identification of four molecular chaperones, in addition to five previously identified chaperone, have provided mechanistic insight into how this cellular megastructure is assembled in the cell.

TL;DR: It is shown that in the yeast Saccharomyces cerevisiae three proteins are found associated with RP but not with the RP–CP holoenzyme: Nas6, Rpn14 and Hsm3 are RP chaperones, and it is shown in an accompanying study that RP assembly is templated through the Rpt C termini, apparently by their insertion into binding pockets in the CP.