Gisèle Laguerre

TL;DR: The results support the view that lateral genetic transfer across rhizobial species and, in some instances, across Rhizobia and Sinorhizobium genera plays a role in diversification and in structuring the natural populations of rhizobia.

TL;DR: This PCR-RFLP method provides a rapid tool for the identification of root nodule isolates and the detection of new taxa.

TL;DR: Thirty-one strains of two new genomic species of rhizobia isolated from root nodules of Phaseolus vulgaris and originating from various locations in France were compared with reference strains by performing a numerical analysis of 64 phenotypic features, revealing that each genomic species formed a lineage independent of the lineages formed by the previously recognized species of Rhizobia.

TL;DR: Characterization of 43 strains of Rhizobium leguminosarum biovars viciae, trifolii, and phaseoli was performed by two methodologies based on PCR amplification, i.e., PCR DNA fingerprinting of interrepeat sequences and restriction fragment length polymorphism (RFLP) analysis of PCR -amplified chromosomal and symbiotic gene regions.

TL;DR: The ability of denaturing gradient gel electrophoresis technique to resolve 16S rDNA products generated from two different collections of bacteria using universal 16S primers was investigated and the difference in sequence divergence within the two groups members allowed therefore to scale the resolution ability of the DGGE technique.