Rapid Identification of Rhizobia by Restriction Fragment Length Polymorphism Analysis of PCR-Amplified 16S rRNA Genes.
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This PCR-RFLP method provides a rapid tool for the identification of root nodule isolates and the detection of new taxa.Abstract:
Forty-eight strains representing the eight recognized Rhizobium species, two new Phaseolus bean Rhizobium genomic species, Bradyrhizobium spp., Agrobacterium spp., and unclassified rhizobia from various host plants were examined by restriction fragment length polymorphism (RFLP) analysis of 16S rRNA genes amplified by polymerase chain reaction (PCR). Twenty-one composite genotypes were obtained from the combined data of the RFLP analysis with nine endonucleases. Species assignments were in full agreement with the established taxonomic classification. Estimation from these data of genetic relationships between and within genera and species correlated well with previously published data based on DNA-rRNA hybridizations and sequence analysis of 16S rRNA genes. This PCR-RFLP method provides a rapid tool for the identification of root nodule isolates and the detection of new taxa. Imagesread more
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Characterization of microbial diversity by determining terminal restriction fragment length polymorphisms of genes encoding 16S rRNA.
TL;DR: Computer-simulated analysis of terminal restriction fragment length polymorphisms (T-RFLP) for 1,002 eubacterial sequences showed that with proper selection of PCR primers and restriction enzymes, 686 sequences could be PCR amplified and classified into 233 unique terminal restriction fragments lengths or "ribotypes."
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Polyphasic taxonomy, a consensus approach to bacterial systematics.
TL;DR: In this review, the practice of polyphasic taxonomy is discussed for four groups of bacteria chosen for their relevance, complexity, or both: the genera Xanthomonas and Campylobacter, the lactic acid bacteria, and the family Comamonadaceae.
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rRNA operon copy number reflects ecological strategies of bacteria.
TL;DR: It is found that the number of rRNA genes correlates with the rate at which phylogenetically diverse bacteria respond to resource availability, and phenotypic effects associated with rRNA gene copy number are indicative of ecological strategies influencing the structure of natural microbial communities.
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Classification of rhizobia based on nodC and nifH gene analysis reveals a close phylogenetic relationship among Phaseolus vulgaris symbionts.
TL;DR: The results support the view that lateral genetic transfer across rhizobial species and, in some instances, across Rhizobia and Sinorhizobium genera plays a role in diversification and in structuring the natural populations of rhizobia.
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Genotypic Microbial Community Profiling: A Critical Technical Review
TL;DR: This document presents and critically compare these methods commonly used for the study of microbial diversity, including amplified ribosomal DNA restriction analysis, automated Ribosomal intergenic spacer analysis, terminal restriction fragment length polymorphism, and denaturing gradient gel electrophoresis.
References
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Journal ArticleDOI
The neighbor-joining method: a new method for reconstructing phylogenetic trees.
Naruya Saitou,Masatoshi Nei +1 more
TL;DR: The neighbor-joining method and Sattath and Tversky's method are shown to be generally better than the other methods for reconstructing phylogenetic trees from evolutionary distance data.
Journal ArticleDOI
16S ribosomal DNA amplification for phylogenetic study.
TL;DR: A set of oligonucleotide primers capable of initiating enzymatic amplification (polymerase chain reaction) on a phylogenetically and taxonomically wide range of bacteria is described in this paper.
Journal ArticleDOI
Mathematical model for studying genetic variation in terms of restriction endonucleases
Masatoshi Nei,Wen-Hsiung Li +1 more
TL;DR: A mathematical model for the evolutionary change of restriction sites in mitochondrial DNA is developed and a measure called "nucleotide diversity" is proposed to express the degree of polymorphism in a population at the nucleotide level.
Journal ArticleDOI
Report of the Ad Hoc Committee on Reconciliation of Approaches to Bacterial Systematics
Lawrence G. Wayne,Don J. Brenner,R. R. Colwell,Patrick A. D. Grimont,O. Kandler,Micah I. Krichevsky,L. H. Moore,W. E. C. Moore,R. G. E. Murray,Erko Stackebrandt,M. P. Starr,H. G. Truper +11 more
Journal ArticleDOI
R factor transfer in Rhizobium leguminosarum.
TL;DR: R factors of the compatibility class P were transferred between strains of Escherichia coli k12 and Rhizobium leguminosarum and conferred similar levels of antibiotic resistance to those in the corresponding R+ E. coli k 12 hosts, with the exception of carbenicillin resistance which was greatly reduced.
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