Gitte Neubauer

TL;DR: The machinery that removes introns from mRNA precursors — the spliceosome — is a large multi-protein complex that is already sufficiently complete to allow rapid characterization of large mammalian protein complexes via mass spectrometry, and this is the first characterization of an entire mammalian multi- protein complex using these methods.

TL;DR: In this article, the IP3R-associated cGMP kinase substrate (IRAG) was identified as an essential NO/cGKI-dependent regulator of IP3-induced calcium release.

TL;DR: The nanoelectrospray ion source (NanoES) developed recently has been an excellent tool for the sequencing of peptides out of unseparated mixtures, but at lower levels of analyte, it is increasingly difficult to distinguish the peptide ions in the spectrum, limiting the overall sensitivity of the procedure.

TL;DR: The results establish a novel role of c-Src kinase in translational gene regulation and reveal a mechanism by which silenced mRNAs can be translationally activated.

TL;DR: The success in determining phosphorylation sites depends crucially on the digestion, extraction, and detection efficiency for individual phosphopeptides, as indicated by the reported attomole sensitivity for the detection of model phosphopes using precursor ion scans.