Gjalt W. Huisman

TL;DR: It is shown that the intermediate 3-Hydroxy fatty acids can also be polymerized to intracellular poly-(R)-3-hydroxyalkanoates (PHAs) when the medium contains limiting amounts of essential elements, such as nitrogen.

TL;DR: Analysis of a P. putida pha mutant and complementation of this mutant with the cloned pha locus revealed that the PHA polymerase genes necessary for PHA synthesis from octanoate are also responsible for P HA formation from glucose.

TL;DR: While the fluorescent pseudomonads are characterized by their inability to make PHB, they appear to share the capacity to produce PHAs, which may be useful in the optimization of PHA production for biopolymer applications.

TL;DR: In this article, a 25-kilobase (kb) DNA fragment was isolated from Pseudomonas oleovorans and used to select DNA fragments which encode poly(3-hydroxyalkanoates) (PHAs) and a PHA depolymerase.

TL;DR: Although PHA synthesis generally begins only after an essential nutrient such as N, P, S or Mg becomes limiting, at least one strain is identified that begins producing PHA during the exponential growth phase, and the PHAs formed were essentially indistinguishable from wild-type PHAs with respect to their thermal characteristics.