There is, I think, something ethereal about i —the square root of minus one. I remember first hearing about it at school. It seemed an odd beast at that time—an intruder hovering on the edge of reality.

Usually familiarity dulls this sense of the bizarre, but in the case of i it was the reverse: over the years the sense of its surreal nature intensified. It seemed that it was impossible to write mathematics that described the real world in …

I and i

Detection of chemical and biological agents plays a fundamental role in biomedical, forensic and environmental sciences1–4 as well as in anti bioterrorism applications.5–7 The development of highly sensitive, cost effective, miniature sensors is therefore in high demand which requires advanced technology coupled with fundamental knowledge in chemistry, biology and material sciences.8–13

In general, sensors feature two functional components: a recognition element to provide selective/specific binding with the target analytes and a transducer component for signaling the binding event. An efficient sensor relies heavily on these two essential components for the recognition process in terms of response time, signal to noise (S/N) ratio, selectivity and limits of detection (LOD).14,15 Therefore, designing sensors with higher efficacy depends on the development of novel materials to improve both the recognition and transduction processes. Nanomaterials feature unique physicochemical properties that can be of great utility in creating new recognition and transduction processes for chemical and biological sensors15–27 as well as improving the S/N ratio by miniaturization of the sensor elements.28

Gold nanoparticles (AuNPs) possess distinct physical and chemical attributes that make them excellent scaffolds for the fabrication of novel chemical and biological sensors (Figure 1).29–36 First, AuNPs can be synthesized in a straightforward manner and can be made highly stable. Second, they possess unique optoelectronic properties. Third, they provide high surface-to-volume ratio with excellent biocompatibility using appropriate ligands.30 Fourth, these properties of AuNPs can be readily tuned varying their size, shape and the surrounding chemical environment. For example, the binding event between recognition element and the analyte can alter physicochemical properties of transducer AuNPs, such as plasmon resonance absorption, conductivity, redox behavior, etc. that in turn can generate a detectable response signal. Finally, AuNPs offer a suitable platform for multi-functionalization with a wide range of organic or biological ligands for the selective binding and detection of small molecules and biological targets.30–32,36 Each of these attributes of AuNPs has allowed researchers to develop novel sensing strategies with improved sensitivity, stability and selectivity. In the last decade of research, the advent of AuNP as a sensory element provided us a broad spectrum of innovative approaches for the detection of metal ions, small molecules, proteins, nucleic acids, malignant cells, etc. in a rapid and efficient manner.37



Figure 1

Physical properties of AuNPs and schematic illustration of an AuNP-based detection system.



In this current review, we have highlighted the several synthetic routes and properties of AuNPs that make them excellent probes for different sensing strategies. Furthermore, we will discuss various sensing strategies and major advances in the last two decades of research utilizing AuNPs in the detection of variety of target analytes including metal ions, organic molecules, proteins, nucleic acids, and microorganisms.

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Gold nanoparticles in chemical and biological sensing.

基礎講座 電気泳動(Electrophoresis)

This protocol provides an introduction to soft lithography--a collection of techniques based on printing, molding and embossing with an elastomeric stamp. Soft lithography provides access to three-dimensional and curved structures, tolerates a wide variety of materials, generates well-defined and controllable surface chemistries, and is generally compatible with biological applications. It is also low in cost, experimentally convenient and has emerged as a technology useful for a number of applications that include cell biology, microfluidics, lab-on-a-chip, microelectromechanical systems and flexible electronics/photonics. As examples, here we focus on three of the commonly used soft lithographic techniques: (i) microcontact printing of alkanethiols and proteins on gold-coated and glass substrates; (ii) replica molding for fabrication of microfluidic devices in poly(dimethyl siloxane), and of nanostructures in polyurethane or epoxy; and (iii) solvent-assisted micromolding of nanostructures in poly(methyl methacrylate).

/pdf/soft-lithography-for-micro-and-nanoscale-patterning-21e1ngmrqv.pdf

Soft lithography for micro- and nanoscale patterning

A review of the substrates used in microbial fuel cells (MFCs) for sustainable energy production.

We report here an approach to rapidly construct organized formations of micron-scale pillars from a thin polydimethylsiloxane (PDMS) film by optically induced electrohydrodynamic instability (OEHI). In OEHI, a heterogeneous electric field is induced across two thin fluidic layers by stimulating a photoconductive thin film in a parallel-plate capacitor configuration with visible light. We demonstrated that this OEHI method could control nucleation sites of pillars formed by electrohydrodynamic instability. To investigate this phenomenon, a tangential electric force component is assumed to have arisen from the surface polarization charge and is introduced into the traditional perfect dielectric model for PDMS films. Numerical simulation results showed that this tangential electric force played an important role in OEHI.

Non-ultraviolet-based patterning of polymer structures by optically induced electrohydrodynamic instability

We present a multipoint “virtual dispenser” to draw femtolitre droplets from a dielectric fluidic thin film using pulse-voltage-triggered optically induced electrohydrodynamic instability (PVT-OEHI). The “virtual dispenser” generates instability nucleation sites by controlling the optically induced lateral electrical stress and thermocapillary flow inside an optoelectronics chip. A time scale analysis shows that the electrohydrodynamic (EHD) instability phenomenon is present; however, its external manifestation is suppressed by OEHI. We observed two droplet dispensing mechanisms which correspond to different EHD states: Taylor cone formation and optically induced EHD jet. The EHD states transition could be realized by adjusting the pulse voltage parameters to alter the morphology of dispensed micron-scale polymer droplets, which could then be formed into organized arrays of microlenses with controllable diameter and curvature based on surface tension effect.

Exploring pulse-voltage-triggered optically induced electrohydrodynamic instability for femtolitre droplet generation

Micro-ElectroMechanical-Systems (MEMS) have emerged as a major enabling technology across the engineering disciplines. In this study, the possibility of applying MEMS to the aerodynamic field was explored. We have demonstrated that microtransducers can be used to control the motion of a delta wing in a wind tunnel and can even maneuver a scaled aircraft in flight tests. The main advantage of using micro actuators to replace the traditional control surface is the significant reduction of radar cross-sections. At a high angle of attack, a large portion of the suction loading on a delta wing is contributed by the leading edge separation vortices which originate from thin boundary layers at the leading edge. We used microactuators with a thickness comparable to that of the boundary layer in order to alter the separation process and thus achieved control of the global motion by minute perturbations.

/pdf/macro-aerodynamic-devices-controlled-by-micro-systems-1pl43aj4df.pdf

Macro aerodynamic devices controlled by micro systems

By applying a MEMS thermal film sensors array on a circular cylinder and Wavelet analysis on the signals obtained, the unsteady behaviors of laminar flow separation from the cylinder were investigated in this study. The sensor signals obtained at θ=85 o revealed that the unsteady behaviors of flow separation are characterized by two time scales, one of which is associated by the frequency of vortex shedding, and the other is at least one order of magnitude longer due to the excursion of flow separation in a circumferential region about 5 o . The Wavelet analysis results enabled one to reduce the percentage of time of which the vortex shedding frequency was detectable at the location measured. It is found that the percentage values are very close to 1 in the region upstream of flow separation, followed by a pronounced transition from θ=85 o to 100 o , at which the percentage values are decreased to about 0.2. Low-frequency variations were noticed in the signals of the thermal films upstream of flow separation, and a hot-wire probe situated in the free stream. Further, a negative correlation between the vortex shedding frequency and its modulus was realized from the hot-wire signals measured in the free stream, inferring that the lower the vortex shedding frequency, the higher the amplitude of the vortex shedding frequency component.

Sensing flow separation on a circular cylinder by MEMS thermal-film sensors

The development of carbon nanotube (CNT)-based sensors remains an active area of research. Towards this end, a new method for manipulating CNTs, assembling CNT networks and fabricating CNT-based nanosensors was demonstrated in this study. CNTs were collected and concentrated by optically-induced dielectrophoresis (ODEP) forces and aligned between a pair of electrodes. This assembly was then used directly as a temperature sensor and a hot-film anemometer, which detects changes in windspeed. By offering efficient CNT collection and ready-to-use sensor fabrication, this ODEP-based approach presents a promising method for the development of CNT-based sensing applications and massively parallel assembly of CNT-lines. The developed CNT-based nanosensors may be used to measure the temperature and the flow velocity of bio-samples in the near future.

Gwo-Bin Lee

Papers

Non-ultraviolet-based patterning of polymer structures by optically induced electrohydrodynamic instability

Exploring pulse-voltage-triggered optically induced electrohydrodynamic instability for femtolitre droplet generation

Macro aerodynamic devices controlled by micro systems

Sensing flow separation on a circular cylinder by MEMS thermal-film sensors

Carbon nanotube-based hot-film and temperature sensor assembled by optically-induced dielectrophoresis.