H
Hidelita Santos
Researcher at Fox Chase Cancer Center
Publications - 4
Citations - 1091
Hidelita Santos is an academic researcher from Fox Chase Cancer Center. The author has contributed to research in topics: Chromatin & Histone code. The author has an hindex of 3, co-authored 4 publications receiving 1045 citations.
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Journal ArticleDOI
Formation of MacroH2A-Containing Senescence-Associated Heterochromatin Foci and Senescence Driven by ASF1a and HIRA
Rugang Zhang,Maxim Poustovoitov,Maxim Poustovoitov,Xiaofen Ye,Hidelita Santos,Wei Chen,Sally M. Daganzo,Sally M. Daganzo,Jan P. Erzberger,Ilya G. Serebriiskii,Adrian A. Canutescu,Roland L. Dunbrack,John R. Pehrson,James M. Berger,Paul D. Kaufman,Paul D. Kaufman,Peter D. Adams +16 more
TL;DR: Analysis of composition and mode of assembly of SAHF indicates that HIRA and ASF1a drive formation of macroH2A-containing SAHF and senescence-associated cell cycle exit, via a pathway that appears to depend on flux of heterochromatic proteins through PML bodies.
Journal ArticleDOI
Defective S Phase Chromatin Assembly Causes DNA Damage, Activation of the S Phase Checkpoint, and S Phase Arrest
Xiaofen Ye,Alexa A. Franco,Alexa A. Franco,Hidelita Santos,David M. Nelson,Paul D. Kaufman,Paul D. Kaufman,Peter D. Adams +7 more
TL;DR: This work used a dominant-negative mutant of a subunit of CAF-I, a complex that assembles newly synthesized DNA into nucleosomes, to inhibit S phase chromatin assembly and found that this induced S phase arrest.
Journal ArticleDOI
Coupling of DNA Synthesis and Histone Synthesis in S Phase Independent of Cyclin/cdk2 Activity
David M. Nelson,Xiaofen Ye,Caitlin Hall,Hidelita Santos,Tianlin Ma,Gary D. Kao,Tim J. Yen,J. Wade Harper,Peter D. Adams +8 more
TL;DR: It is shown that ectopic expression of HIRA, the likely human ortholog of two cell cycle-regulated repressors of histone gene transcription in yeast, represses transcription of histones and that this, in turn, triggers a concerted block of DNA synthesis.
Book ChapterDOI
Biochemical analysis of the cell cycle and cell cycle checkpoints in transiently transfected cells after collection with magnetic beads.
TL;DR: It is frequently necessary to examine the biochemical effects of ectopically expressed proteins or short hairpin (sh) RNA-mediated protein knock-down in intact cells, and the CD19/CD20 cell surface marker approach can be readily combined with analysis of cell cycle distribution of propidium-iodide-stained cells to simultaneously determine the biochemical and cell cycle effects.