Hiroki Maeda

TL;DR: Advances in the development of 4,4-difluoro-4-bora-3a,4a-diaza-s-indacene (BODIPY)-based fluorescent probes for biological studies over the past decade are covered.

TL;DR: Spatiotemporal intercellular interactions between mOBs and mOCs affecting bone homeostasis in vivo are revealed, by using an intravital imaging technique, that mOB and m OC functions are regulated via direct cell–cell contact between these cell types.

TL;DR: P pH-activatable chemical probes BAp-M andBAp-E have been developed and will prove to be a powerful tool for the selective detection of bone-resorbing osteoclasts in vivo, because this is the first instance where in vivo imaging has been conducted in a low-pH region created by bone- Resorbing fractures.

TL;DR: A functional SMP with an active-targeting moiety, higher photostability, and a fluorescence switch is designed and developed and then imaged target cell activity by injecting the SMP into living mice and real-time imaging revealed heterogenic behaviors of osteoclasts in vivo and provided insights into the mechanism of bone resorption.

TL;DR: A rhodamine-based small-molecule fluorescent probe called Red-pHocas is developed to reversibly detect the acidic environments for the spatiotemporal analysis of the function of osteoclast proton pumps in bone acidification under intravital conditions by means of an imaging probe.