Hokyung K. Chung

TL;DR: Light-dependent dissociation and association in a mutant of the photochromic FP Dronpa is described and used to control protein activities with light, extending the applications of FPs from exclusively sensing functions to also encompass optogenetic control.

TL;DR: Small Molecule-Assisted Shutoff (SMASh), a technique in which proteins are fused to a degron that removes itself in the absence of drug, leaving untagged protein, is described.

TL;DR: This design successfully controlled different species and functional variants of Cas9, mediated transcriptional activation more robustly than previous optogenetic methods, and enabled light-induced transcription of one gene and editing of another in the same cells.

TL;DR: An RSV strain carrying firefly luciferase fused to an innovative universal small-molecule assisted shut-off domain, which boosted assay signal window, and a hyperactive fusion protein that synchronized IAV and RSV reporter expression kinetics and suppressed the identification of RSV entry inhibitors sensitive to a recently reported RSV pan-resistance mechanism are developed.

TL;DR: Rewiring of Aberrant Signaling to Effector Release (RASER), in which a compact synthetic signaling pathway detects an oncogenic signal with high specificity and then rewires it to a variety of customizable responses.