Hong-Mei Han

TL;DR: The use of cryo FIB-SEM is described as a tool for direct and fast 3D cryo-imaging of large native frozen samples including tissues.

TL;DR: PM-proximal sub-regions of the ER are identified as important sites of cellular signaling regulation by PTP1B, and the ER anchor plays an important role in restricting its interactions with PM substrates mainly to regions of cell-cell contact.

TL;DR: Using high-resolution cryo-imaging, it is shown that cells tolerate crystallization of extra- and intracellular water, which suggests that cryoprotectants can act by inhibiting crystallization or recrystallization, but they also increase the tolerance toward ice-crystal growth.

TL;DR: The quality of results is equivalent to samples prepared by conventional high pressure freezing apparatus, thus establishing this novel method as fast, easy-to-use and low-cost freeze fixation alternative for cryo-EM.

TL;DR: The presence of intracellular membrane structures in spores located just below the core membrane is demonstrated, demonstrating the presence of endospore forming bacteria with particular architecture and molecular inventory with remarkable resistance against desiccation, heat and radiation.