Jacob Krüger Jensen

TL;DR: The largely unused uracil-excision molecular cloning technique is advanced by identifying PfuCx as a compatible proof-reading DNA polymerase and by developing an improved vector design strategy.

TL;DR: Biochemical methods successfully led to the recent identification of a pectin biosynthetic galacturonosyltransferase (GAUT1), and recent functional genomics and mutant studies have allowed the identification of several biosynthetics enzymes involved in making different parts of pectIn several other cases, enzymes have been identified as involved inMaking pect in but because of ambiguity in the cell wall compositions of mutants and lack of direct biochemical evidence their specific activities are more uncertain.

TL;DR: Analysis of mutant lines with T-DNA insertion in the ARABINAN DEFICIENT 1 gene suggested that ARAD1 is an arabinan α-1,5-arabinosyltransferase, a putative glycosyl transferase of type 2 membrane protein with a signal anchor in Arabidopsis.

TL;DR: Using pyrosequencing, over 7 million ESTs from four stages of developing seeds of Ricinus communis, Brassica napus, Euonymus alatus and Tropaeolum majus are generated, revealing both conserved and distinct species-specific expression patterns for genes involved in the synthesis of glycerolipids and their precursors.

TL;DR: Results confirm that the XGD1 protein is a XGA xylosyltransferase, shown by expression of a fluorescent fusion protein in N. benthamiana to be localized in the Golgi vesicles as expected for a glycosyl transferase involved in pectin biosynthesis.