J
James E. Brownell
Researcher at University of Virginia
Publications - 5
Citations - 1558
James E. Brownell is an academic researcher from University of Virginia. The author has contributed to research in topics: Histone acetyltransferase & NEDD8. The author has an hindex of 5, co-authored 5 publications receiving 1497 citations.
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Journal ArticleDOI
Yeast Gcn5 functions in two multisubunit complexes to acetylate nucleosomal histones: characterization of an Ada complex and the SAGA (Spt/Ada) complex.
Patrick A. Grant,Laura J. Duggan,Jacques Côté,S M Roberts,James E. Brownell,Reyes Candau,Reiko Ohba,Tom Owen-Hughes,C D Allis,Fred Winston,Shelley L. Berger,Jerry L. Workman +11 more
TL;DR: The function of Gcn5 as a hist one acetyltransferase within the Ada and SAGA adaptor complexes indicates the importance of histone acetylation during steps in transcription activation mediated by interactions with transcription activators and general transcription factors.
Journal ArticleDOI
Nedd8 Modification of Cul-1 Activates SCF β TrCP -Dependent Ubiquitination of IκBα
Margaret A. Read,James E. Brownell,Tatiana B. Gladysheva,Maria Hottelet,Lana A. Parent,Michael B. Coggins,Jacqueline W. Pierce,Vladimir N. Podust,Vladimir N. Podust,Rong Shu Luo,Vincent Chau,Vito J. Palombella +11 more
TL;DR: Recently, the receptor component of the IκB E3 was identified as a member of the βTrCP (beta-transducin repeat-containing protein) family of proteins called E3RSIκB (39, 53, 63, 65) or HOS (11).
Book ChapterDOI
Histone acetyltransferases: preparation of substrates and assay procedures.
TL;DR: The methods described in this chapter should be efficacious for the detection of acetyltransferases specific for nonhistone substrates and should facilitate the investigation of the role(s) of protein ɛ-N-acetylation in nuclear function.
Journal ArticleDOI
A novel H2A/H4 nucleosomal histone acetyltransferase in Tetrahymena thermophila.
Reiko Ohba,David J. Steger,James E. Brownell,Craig A. Mizzen,Richard G. Cook,Jacques Côté,Jerry L. Workman,C. David Allis +7 more
TL;DR: Together, these data document the importance of this novel HAT activity for transcriptional activation from chromatin templates and suggest that a second catalytic HAT subunit, in addition to p55/Gcn5p, is conserved between yeast and Tetrahymena.
Ubiquitination of IkBa
Margaret Read,James E. Brownell,Tatiana B. Gladysheva,Maria Hottelet,Lana A. Parent,Michael B. Coggins,Jacqueline W. Pierce,Vladimir N. Podust,Rong-Shu Luo,Vincent Chau,Vito Palombella +10 more
TL;DR: A functional link between the highly related ubiquitin and Nedd8 pathways of protein modification is provided and how they operate together to selectively target the signal-dependent degradation of IkBa is shown.