James H. Gerlach

TL;DR: In this paper, the intron-exon structures of TOP2A and TOP2B have been defined and the amino-acid sequences of the two proteins were aligned, showing that the two genes are highly conserved except for the regions encoding the extreme NH2 and COOH termini of the proteins.

TL;DR: The results confirm that a broader definition is required to detect all potential bipartite NLS motifs in a polypeptide sequence, although functional tests are still essential for identification of those sequences actually capable of directing nuclear localization.

TL;DR: It is shown that human topo IIalpha tagged with green fluorescence protein is still detectable in the nucleus when alphaNLS(1454-1497) has been disrupted, and differences in the NLS sequences of humanTopo II isoforms may contribute to their differences in subnuclear localization.

TL;DR: The remarkably high levels and frequency of MRP expression in some SCLC samples indicate that future developments in chemotherapy of this tumour type should anticipate that drugs which are substrates of MRp may be of limited effectiveness.

TL;DR: A semi-quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) assay to measure levels of topo II α and β mRNAs simultaneously using a single pair of primers with sequences common to both isoforms is developed, and for the first time, makes the rapid simultaneous direct comparison oftopo IIα and topoIIβ mRNA levels feasible in large numbers of clinical samples.