Showing papers in "Gene in 1998"

TL;DR: In this article, three classes of GFP mutants having single excitation maxima around 488 nm are shown to be brighter than wild-type GFP following 488-nm excitation.

TL;DR: An improved method for gene replacement in Pseudomonas aeruginosa was developed and a cassette was constructed that contains a GmR selectable marker next to the green fluorescent protein structural gene, with both markers being flanked by Flp recombinase target (FRT) sites.

TL;DR: Regulation of 987P fimbriation could be identified after replacement of fasA and fasH with allelic reporter fusions and expression is not dependent on the exogenous iron concentration.

TL;DR: The existence of naturally occurring antisense RNAs has been illustrated, in eukaryotes, by an increasing number of reports as discussed by the authors, with a special focus on the regulation of gene expression exerted by endogenous complementary transcripts.

TL;DR: Two loxP mutants with double-base substitutions are identified, mutants 5171 and 2272, which recombine efficiently with an identical mutant but not with the other mutant or wild-type lox P.

TL;DR: The poly(A)-binding protein (PABP) interacts with the cap-associated eukaryotic initiation factor (eIF) 4G (in yeast and plants) and eIF4B (in plants) as discussed by the authors.

TL;DR: The HBD-2 gene is the first member of the human defensin family that is locally inducible by inflammation and located H BD-2 as the most centromeric of the genes.

TL;DR: It is found that the previously described localization of wild-type GFP to the cell nucleus is most likely due to diffusion of GFP across the nuclear envelope rather than to a cryptic nuclear localization signal.

TL;DR: The phiPVL genome was found to integrate into an ORF encoding an unknown protein comprising 725 amino acid residues with two leucine zipper-like motifs and showed a high degree of homology with the recombinase-binding sites of some other S. aureus bacteriophages.

TL;DR: Low-density lipoprotein receptor related protein 5 (LRP5) was identified by DNA sequence analysis within the insulin-dependent diabetes mellitus (IDDM) locus IDDM4 on chromosome 11q13 as discussed by the authors.

TL;DR: Human cGMP-binding, cG MP-specific 3',5'-cyclic nucleotide phosphodiesterase (PDE5A) cDNAs were isolated and a 5'-splice variant, PDE5A2, encodes an 833-amino-acid protein with eight unique amino acids at the amino terminus.

TL;DR: A series of vectors is described which enables the episomal expression of proteins fused to different tag sequences in Schizosaccharomyces pombe, controlled by the medium strength mutant version of the regulatable nmt1 promoter.

TL;DR: The pET system, in which the phage T7 RNA polymerase gene is expressed via lac operon control elements, exhibits leaky expression that increases markedly as cells grown in complex medium enter stationary phase, and it is found that this phenomenon occurs with the chromosomal Lac operon as well.

TL;DR: This is the first report showing that leptin gene expression in chicken is not exclusively localized in adipose tissue but is also expressed in liver, and the expression of leptin in liver may be associated with a key role of this organ in avian species in controlling lipogenesis.

TL;DR: The Tol2 element is capable of excision in zebrafish embryos, presumably dependent on a putative transposase encoded by the Tol1 element itself.

TL;DR: The results show that the C-probe was 1000 times more sensitive than the corresponding linear hemiprobes for detecting Epstein-Barr virus early RNA and increases the power of amplification but also offers a means for decontaminating carryover amplicons.

TL;DR: The skewed oligomer method is shown to work for all bacterial genomes and one of three archaeal genomes sequences to date, confirming known or predicted origins in most cases and in three cases suggesting origins that were previously unknown.

TL;DR: The cloning and characterization of human PON2, a paraoxonase-related gene-2 that is physically linked with PON1 and PON3 on 7q2l is reported and two polymorphisms in the coding sequences that, in the protein deduced from the longest open reading frame, predict an alanine-to-glycine substitution at residue 147 and a serine- to-cysteine substitutions at residue 310 are described.

TL;DR: Investigation of the relationship between codon usage and the physiological pattern of expression of a gene is investigated while considering a dataset of 815 nuclear genes of Arabidopsis thaliana and reveals a single major trend inCodon usage among genes inArabidopsis.

TL;DR: It is shown that the FLD1 promoter (PFLD1) is strongly and independently induced by either methanol as sole carbon source or methylamine as sole nitrogen source, allowing the investigator a choice of carbon or nitrogen source (methylamine) regulation with the same expression strain.

TL;DR: A novel approach in molecular design is presented, where in vivo formed complementarity determining regions (CDR) from antibody genes were shuffled into a specific framework region to create a synthetic gene library of soluble VH-fragments.

TL;DR: The HCV core protein appears to act as an effector in the promotion of cell growth by repressing p21 transcription through unknown cellular factor(s).

TL;DR: In this paper, the 5' Cbfa1/Osf2 sequence is encoded by a previously unrecognized upstream exon, designated exon -1, which is highly conserved in mouse, rat and human.

TL;DR: A full-length cDNA clone encoding a novel LIM-only protein was isolated and sequenced from a human fetal heart cDNA library and no signal could be detected in brain, lung, liver, kidney, pancreas, spleen, thymus, prostate, testis, small intestine, colon or peripheral blood leukocyte.

TL;DR: The eukaryotic organization of the four cyst nematode cellulases suggests that they share a common ancestor, and their strong homology to prokaryotic glycosyl hydrolases may be indicative of an ancient horizontal gene transfer.

TL;DR: The Wnt gene family encodes a set of signalling molecules, thought to play an important role in key processes of embryonic development, and its expression in the perichondrium of the developing skeleton makes it a plausible candidate gene for HBM, which has been previously linked to markers from this region.

TL;DR: Ten new sheep genes which encode potential antimicrobial peptides including two beta-defensins and eight cathelicidins are identified and sequenced, confirming homology between sheep, cattle, mouse, and human antimacterial peptide gene families.

TL;DR: An improved algorithm for the simultaneous alignment of multiple protein and nucleic acid sequences, the Divide-and-Conquer Alignment procedure (DCA), is presented and several examples are presented showing that the program is able to deal with real-world alignment problems.

TL;DR: A Gram-positive cloning vector that utilizes the interruption of an alkaline phosphatase gene, phoZ, to identify recombinant plasmids is developed and should improve the efficiency of performing recombinant DNA experiments in Gram- positive bacteria.

TL;DR: Western analysis suggests that the CSHMT gene is expressed as a single full-length protein in 5Y cells, but as multiple forms in MCF-7 cells, and multiple tissue Northern blots suggest that theCSHMT message levels and alternative splicing patterns display tissue-specific variations.