Jingyi Huang

TL;DR: This work presents a general strategy to covalently link a synthetic organic fluorophore simultaneously to a photostabilizer and biomolecular target via unnatural amino acids, and is convinced that the presented scaffolding strategy and the improved characteristics of the conjugates in applications will trigger the broader use of intramolecularPhotostabilization and help to emerge this approach as a new gold standard.

TL;DR: It is shown that a covalently coupled photostabilizer can replace a mixture of molecules needed to make a functionalPhotostabilizing ROXS buffer and might hence represent the new standard for defined and reproducible imaging conditions in single-molecule experiments.

TL;DR: It is found that although the rate of triplet-state quenching is additive, the photostability is limited by the faster pathway, and intramolecular photostabilizers can protect fluorophores from reversible off-switching events caused by solution-additives, which was previously misinterpreted as photobleaching.

TL;DR: A trans encoded ribo-switch is reported, which consists of an engineered tRNA mimicking structure (TMS), under control of small photo-switchable signalling molecules, which is believed to provide a powerful platform for controlling the expression of other functional proteins with high spatiotemporal resolution employing light as a stimulus.

TL;DR: This corrects the article DOI: 10.1038/ncomms10144 to indicate that the author of the paper is a doctor rather than a scientist, as previously reported.