Silvia Galiani

TL;DR: This work suggests that incipient signaling in T cells initiates global cytoskeletal rearrangements across the whole cell, including a stiffening process for possibly mechanically supporting contact formation at the immunological synapse interface as well as a central ramified transportation network apparently directed at the consolidation of the contact and the delivery of effector functions.

TL;DR: The diffusion dynamics of lipids and GPI-anchored proteins is investigated using superresolution STED microscopy combined with single-molecule fluorescence correlation spectroscopy in the cellular membranes.

TL;DR: A simple theoretical framework is presented that help to explain the influence of excitation and STED pulses and their influence on the performance of a STED microscope and the advantages of using time gating detection in terms of temporal alignment.

TL;DR: This work presents a general strategy to covalently link a synthetic organic fluorophore simultaneously to a photostabilizer and biomolecular target via unnatural amino acids, and is convinced that the presented scaffolding strategy and the improved characteristics of the conjugates in applications will trigger the broader use of intramolecularPhotostabilization and help to emerge this approach as a new gold standard.

TL;DR: It is shown that a widely used red-emitting fluorophore, ATTO647N, can be two-photon excited at a wavelength allowing both 2PE and STED using the very same laser source, opening the possibility to perform 2PE microscopy at four to five times STED-improved resolution, while exploiting the intrinsic advantages of nonlinear excitation.