Jochem H. Smit

TL;DR: This work presents a general strategy to covalently link a synthetic organic fluorophore simultaneously to a photostabilizer and biomolecular target via unnatural amino acids, and is convinced that the presented scaffolding strategy and the improved characteristics of the conjugates in applications will trigger the broader use of intramolecularPhotostabilization and help to emerge this approach as a new gold standard.

TL;DR: The relation of OFF-state lifetimes to redox potentials might enable predictions about the nature of dark states, depending on the fluorophores' nano-environment in super-resolution microscopy.

TL;DR: It is shown that a covalently coupled photostabilizer can replace a mixture of molecules needed to make a functionalPhotostabilizing ROXS buffer and might hence represent the new standard for defined and reproducible imaging conditions in single-molecule experiments.

TL;DR: It is found that although the rate of triplet-state quenching is additive, the photostability is limited by the faster pathway, and intramolecular photostabilizers can protect fluorophores from reversible off-switching events caused by solution-additives, which was previously misinterpreted as photobleaching.

TL;DR: It is found that intramolecular photostabilization strongly influences photoswitching kinetics and requires careful attention when designing STORM-experiments, and the superior photophysical performance resulted in optimal STED imaging.