John M. Lawrence

TL;DR: It is demonstrated that the DNA prime-protein boost approach is an effective immunization method to elicit both humoral and cell-mediated immune responses in humans, and that a polyvalent Env formulation could generate broad immune responses against HIV-1 viruses with diverse genetic backgrounds.

TL;DR: The results suggest that Env DNA priming followed by gp120 protein boosting provides an advantage over either approach alone for generating a detectable neutralizing antibody response against primary isolates that are not easily neutralized.

TL;DR: The codon-optimized S gene of SARS-CoV was synthesized to construct DNA vaccine plasmids expressing either the full-length or segments of the S protein, which plays important roles in viral pathogenesis and potentially in the development of an effective vaccine against this virulent infectious disease.

TL;DR: It is demonstrated that the inactivated SARS-CoV was able to preserve the immunogenicity of S protein including its major neutralizing domain, which supports that subunit vaccination with S constructs may also be able to protect animals and perhaps humans.