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Jonathan C. Cruz

Researcher at Dartmouth College

Publications -  6
Citations -  608

Jonathan C. Cruz is an academic researcher from Dartmouth College. The author has contributed to research in topics: Chinese hamster ovary cell & Cholesterol. The author has an hindex of 5, co-authored 6 publications receiving 582 citations. Previous affiliations of Jonathan C. Cruz include Salk Institute for Biological Studies.

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Role of Niemann-Pick Type C1 Protein in Intracellular Trafficking of Low Density Lipoprotein-derived Cholesterol *

TL;DR: It is found that the initial movement of low density lipoprotein (LDL)-derived cholesterol to the plasma membrane (PM) did not require NPC1, but after reaching the PM and subsequent internalization, cholesterol trafficking back to the PM did involve NPC1.
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Niemann-Pick Type C Disease and Intracellular Cholesterol Trafficking

TL;DR: This work presents a novel and scalable approach to gene expression engineering that allows for real-time measurement of the activity of T. Y. Chang’s Tournaisian factor, which is important for understanding the immune response to infectious disease.
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Recombinant acyl-CoA:cholesterol acyltransferase-1 (ACAT-1) purified to essential homogeneity utilizes cholesterol in mixed micelles or in vesicles in a highly cooperative manner.

TL;DR: The hypothesis that ACAT is an allosteric enzyme regulated by cholesterol is supported, as the cholesterol substrate saturation curves of the enzyme assayed either in mixed micelles or in reconstituted vesicles are both highly sigmoidal.
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Fate of endogenously synthesized cholesterol in Niemann-Pick type C1 cells.

TL;DR: Results using the inhibitorN-butyldeoxynojirimycin, which depletes cellular complex glycosphingolipids, demonstrates that the cholesterol trafficking defect in NPC1 cells is not caused by ganglioside accumulation.
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Synthesis and biochemical properties of a new photoactivatable cholesterol analog 7,7-azocholestanol and its linoleate ester in Chinese hamster ovary cell lines.

TL;DR: Results demonstrate AC as an effective reagent for studying cholesterol-protein interactions involved in intracellular cholesterol trafficking as well as examining the biochemical properties of the sterol and its ester by employing several different mutant Chinese hamster ovary cell lines with defined abnormalities in cholesterol metabolism as tools.