Showing papers by "Jörg Peter Kutter published in 2020"
TL;DR: The rapid prototyping possibility combined with the material's resulting mechanical strength, solvent resistance, and biocompatibility, as well as the inherently easy surface functionalization are strong factors to make thiol-ene polymers strong contenders for promising future materials for many biological, clinical and technical lab-on-a-chip applications.
Abstract: While there is a steady growth in the number of microfluidics applications, the search for an optimal material that delivers the diverse characteristics needed for the numerous tasks is still nowhere close to being settled. Often overlooked and still underrepresented, the thiol-ene family of polymer materials has an enormous potential for applications in organs-on-a-chip, droplet productions, microanalytics, and point of care testing. In this review, the main characteristics of the thiol-ene materials are given, and advantages and drawbacks with respect to their potential in microfluidic chip fabrication are critically assessed. Select applications, which exploit the versatility of the thiol-ene polymers, are presented and discussed. It is concluded that, in particular, the rapid prototyping possibility combined with the material's resulting mechanical strength, solvent resistance, and biocompatibility, as well as the inherently easy surface functionalization, are strong factors to make thiol-ene polymers strong contenders for promising future materials for many biological, clinical, and technical lab-on-a-chip applications.
60 citations
TL;DR: This study presents the development and application of a free-flow electrophoresis microfluidic chip, and its upstream combination with an enzyme microreactor with immobilized pepsin in the same miniaturized platform.
Abstract: One of the most attractive aspects of microfluidic chips is their capability of integrating several functional units into one single platform. In particular, enzymatic digestion and chemical separation are important steps in processing samples for many biochemical assays. This study presents the development and application of a free-flow electrophoresis microfluidic chip, and its upstream combination with an enzyme microreactor with immobilized pepsin in the same miniaturized platform. The whole microfluidic chip was fabricated by making use of thiol-ene click chemistry. As a proof of concept, different fluorescent dyes and labeled amino acids were continuously separated in the 2D electrophoretic channel. The protease pepsin was immobilized using a covalent linkage with ascorbic acid onto a high-surface monolithic support, also made of thiol-ene. To show the potential of the microfluidic chip for continuous sample preparation and analysis, an oligopeptide was enzymatically digested, and the resulting fragments were separated and collected in a single step (prior to mass spectrometric detection), without the need of further time-consuming liquid handling steps.
16 citations
TL;DR: A thiol-ene microfluidic chip capable of fast chromatographic sample clean-up, concentration, and separation of complex protein and peptide mixtures with direct on-chip ESI is described.
12 citations
TL;DR: This review summarizes recent developments in the field of microfluidics‐based solutions for enantiomeric separation and detection, covering chiral stationary phases, surface coatings, and modification strategies to improve resolution.
Abstract: This review summarizes recent developments (over the past decade) in the field of microfluidics-based solutions for enantiomeric separation and detection. The progress in various formats of microchip electrodriven separations, such as MCE, microchip electrochromatography, and multidimensional separation techniques, is discussed. Innovations covering chiral stationary phases, surface coatings, and modification strategies to improve resolution, as well as integration with detection systems, are reported. Finally, combinations with other microfluidic functional units are also presented and highlighted.
9 citations
TL;DR: It is demonstrated that inhalable Azt/Tob dry powders using L-leucine as a moisture protector as well as a dispersing agent can be readily prepared by the spray drying process and may represent an alternative treatment against multidrug-resistant Gram-negative respiratory pathogens.
7 citations
TL;DR: An enhanced antibacterial activity of T AP was observed with an increase in the dissolution rate of TAP from the dry powders at certain concentration ranges.
5 citations
01 Jan 2020
TL;DR: This work reports on a novel method for the generation of mitochondria under physiologically relevant conditions that allows the study of cells under physiological relevant conditions.
Abstract: The precise control of the oxygen concentration in a cellular environment allows the study of cells under physiologically relevant conditions. This work reports on a novel method for the generation ...
1 citations
Patent•
23 Jan 2020
TL;DR: A post-polymerization annealing of a thermoset polymer containing a plurality of sulfur atoms is described in this paper. But this method is not suitable for the treatment of polymeric polymers.
Abstract: A method for treatment by post-polymerization annealing of a thermoset polymer, the method comprising the steps of: i) providing a polymerised and cured thermoset polymer containing a plurality of sulfur atoms; and ii) subjecting the cured thermoset polymer to one of the following processes: a) heating the cured thermoset polymer to a temperature of from 50°C to 250°C, and maintaining the cured thermoset polymer at a temperature of from 50°C to 250°C for a period of from 1 minute to 72 hours in an oxygen-containing environment; or b) exposing the cured thermoset polymer to UV radiation of wavelength from 10nm to 400nm at an intensity of from 50 mW/cm2 to 150 m W/cm2 for a period of from 1 minute to 24 hours; and/or c) contacting the cured thermoset polymer with an oxidising agent for a period of from 10 minutes to 24 hours.