Josef Ehgartner

TL;DR: It is concluded that the perfused liver organoid shares relevant morphological and functional characteristics with the human liver and represents a new in vitro research tool to study human hepatocellular physiology at the cellular level under conditions close to the physiological situation.

TL;DR: A microfluidic device is developed for in-situ analysis of pesticide concentration detected via metabolism/photosynthesis of Chlamydomonas reinhardtii algal cells (algae) in tap water and it provides fast and complementary detection of different pesticides with algae in a novel glass based microfluidity device with integrated optical pH, oxygen sensors and algal fluorescence.

TL;DR: Two different microfluidic devices containing embedded sensor arrays are developed to monitor local oxygen levels to investigate oxygen consumption rates of 2D and 3D hydrogel-based cell cultures and the establishment of oxygen gradients within cell culture chambers, and how dynamic control of cyclic normoxic-hypoxic cell microenvironments can be readily accomplished using programmable flow profiles employing both gas-impermeable and gas- permeable microfluidity biochips.

TL;DR: An approach based on continuous carrier oil recirculation that ensures enhanced and homogeneous oxygen availability during mid and long-term incubation of picoliter droplets retained in a 3D-printed storage device is described and validated, substantially improves the robustness and versatility of droplet-based cell assays.

TL;DR: In this article, a powerful online analysis set-up for oxygen measurements within microfluidic devices is presented, which features integration of optical oxygen sensors into microreactors, enabling contactless, accurate and inexpensive readout using commercially available oxygen meters via luminescent lifetime measurements in the frequency domain (phase shifts).