J
Josh T. Cuperus
Researcher at University of Washington
Publications - 44
Citations - 3326
Josh T. Cuperus is an academic researcher from University of Washington. The author has contributed to research in topics: Gene & Biology. The author has an hindex of 16, co-authored 36 publications receiving 2715 citations. Previous affiliations of Josh T. Cuperus include Oregon State University & Howard Hughes Medical Institute.
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Journal ArticleDOI
Specificity of ARGONAUTE7-miR390 interaction and dual functionality in TAS3 trans-acting siRNA formation
Taiowa A. Montgomery,Miya D. Howell,Josh T. Cuperus,Dawei Li,Jesse E. Hansen,Amanda L. Alexander,Elisabeth J. Chapman,Noah Fahlgren,Edwards Allen,James C. Carrington +9 more
TL;DR: It is shown that miR390-ARGONAUTE7 complexes function in distinct cleavage or noncleavage modes at two target sites in TAS3a transcripts, evolving as a highly specific miRNA guide/effector protein pair to function at two distinct tasiRNA biogenesis steps.
Journal ArticleDOI
Evolution and Functional Diversification of MIRNA Genes
TL;DR: Genome-wide analyses from several plant species have revealed that variation in miRNA foldback expression, structure, processing efficiency, and miRNA size have resulted in the unique functionality of MIRNA loci and resulting miRNAs.
Journal ArticleDOI
Unique functionality of 22-nt miRNAs in triggering RDR6-dependent siRNA biogenesis from target transcripts in Arabidopsis
Josh T. Cuperus,Alberto Carbonell,Noah Fahlgren,Hernan Garcia-Ruiz,Russell T. Burke,Atsushi Takeda,Atsushi Takeda,Christopher M. Sullivan,Sunny D. Gilbert,Taiowa A. Montgomery,Taiowa A. Montgomery,James C. Carrington +11 more
TL;DR: Data suggest that AGO1 functions differentially with 21- and 22-nt miRNAs to engage the RDR6-associated amplification apparatus, which is likely to trigger RDR 6-dependent siRNA production from target RNA.
Journal ArticleDOI
Functional Analysis of Three Arabidopsis ARGONAUTES Using Slicer-Defective Mutants
Alberto Carbonell,Noah Fahlgren,Hernan Garcia-Ruiz,Kerrigan B. Gilbert,Taiowa A. Montgomery,Tammy Nguyen,Josh T. Cuperus,James C. Carrington +7 more
TL;DR: Functional roles for AGO1, AGO2, and AGO7 slicer activity are revealed and an approach to capture ternary complexes more efficiently for genome-wide analyses is indicated.
Journal ArticleDOI
Dynamics of Gene Expression in Single Root Cells of Arabidopsis thaliana
Ken Jean-Baptiste,José L. McFaline-Figueroa,Cristina M. Alexandre,Michael W. Dorrity,Lauren M. Saunders,Kerry L. Bubb,Cole Trapnell,Stanley Fields,Christine Queitsch,Josh T. Cuperus +9 more
TL;DR: The results demonstrate that single cell transcriptomics holds promise for studying plant development and plant physiology with unprecedented resolution and address the longstanding question of possible heterogeneity among cell types in the response to an abiotic stress.