Julia Joung

TL;DR: A novel multiplexed RT-LAMP approach is described and validated, which allows for fast and reliable identification of infected individuals and reveals one-step multiplexing RT- LAMP assays as a prime-option for the development of easy and cheap POC test kits.

TL;DR: A large-scale CRISPR screen employing ~18,000 sgRNAs targeting >700 kb of noncoding sequence in an unbiased manner surrounding three genes involved in resistance to the BRAf inhibitor vemurafenib in the BRAF-mutant melanoma cell line A375 is developed.

TL;DR: A CRISPR-based method that uses catalytically active Cas9 and distinct single guide (sgRNA) constructs to knock out and activate different genes in the same cell, without inducing double-stranded breaks is developed.

TL;DR: This review will highlight groundbreaking studies in the CRISPR-Cas9 functional genetics field and discuss strengths and limitations of this technology for neurological disease applications and provide practical guidance on navigating the many choices that need to be made when implementing a CRISpr- Cas9 functional genetic screen for the study of neurological diseases.

TL;DR: In this article, the authors performed whole-genome and transcriptome sequencing, and a genome-wide CRISPR-Cas9-knockout screen to study an activated B-cell-like DLBCL cell line (RC-K8).