J
Jason Wright
Researcher at Broad Institute
Publications - 21
Citations - 10480
Jason Wright is an academic researcher from Broad Institute. The author has contributed to research in topics: Gene & CRISPR. The author has an hindex of 14, co-authored 21 publications receiving 8441 citations. Previous affiliations of Jason Wright include Massachusetts Institute of Technology & Dartmouth College.
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Journal ArticleDOI
Genome engineering using the CRISPR-Cas9 system
F. Ann Ran,Patrick D. Hsu,Jason Wright,Vineeta Agarwala,Vineeta Agarwala,David A. Scott,Feng Zhang +6 more
TL;DR: A set of tools for Cas9-mediated genome editing via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, as well as generation of modified cell lines for downstream functional studies are described.
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Breast cancer risk–associated SNPs modulate the affinity of chromatin for FOXA1 and alter gene expression
Richard Cowper-Sal·lari,Xiaoyang Zhang,Jason Wright,Swneke D. Bailey,Swneke D. Bailey,Michael D. Cole,Jérôme Eeckhoute,Jérôme Eeckhoute,Jason H. Moore,Mathieu Lupien,Mathieu Lupien +10 more
TL;DR: The results show that breast cancer risk–associated SNPs are enriched in the cistromes of FOXA1 and ESR1 and the epigenome of histone H3 lysine 4 monomethylation in a cancer- and cell type–specific manner.
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Genome-scale activation screen identifies a lncRNA locus regulating a gene neighbourhood
Julia Joung,Jesse M. Engreitz,Silvana Konermann,Omar O. Abudayyeh,Vanessa Verdine,Vanessa Verdine,François Aguet,Jonathan S. Gootenberg,Neville E. Sanjana,Jason Wright,Jason Wright,Jason Wright,Charles P. Fulco,Charles P. Fulco,Yuen-Yi Tseng,Charles H. Yoon,Jesse S. Boehm,Eric S. Lander,Eric S. Lander,Eric S. Lander,Feng Zhang +20 more
TL;DR: A genome-scale CRISPR-Cas9 activation screen that targets more than 10,000 lncRNA transcriptional start sites to identify noncoding loci that influence a phenotype of interest found 11 lncRNAs that, upon recruitment of an activator, mediate resistance to BRAF inhibitors in human melanoma cells.
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Upregulation of c-MYC in cis through a Large Chromatin Loop Linked to a Cancer Risk-Associated Single-Nucleotide Polymorphism in Colorectal Cancer Cells
TL;DR: The findings of these studies support a mechanism for intergenic SNPs that can promote cancer through the regulation of distal genes by utilizing preexisting large chromatin loops.
Journal ArticleDOI
High-resolution interrogation of functional elements in the noncoding genome
Neville E. Sanjana,Neville E. Sanjana,Jason Wright,Jason Wright,Kaijie Zheng,Kaijie Zheng,Ophir Shalem,Ophir Shalem,Pierre Fontanillas,Julia Joung,Julia Joung,Christine S. Cheng,Christine S. Cheng,Aviv Regev,Aviv Regev,Feng Zhang,Feng Zhang +16 more
TL;DR: A CRISPR screen using ~18,000 single guide RNAs targeting >700 kilobases surrounding the genes NF1, NF2, and CUL3, which are involved in BRAF inhibitor resistance in melanoma, finds that noncoding locations that modulate drug resistance also harbor predictive hallmarks ofnoncoding function.