K
Kaijie Zheng
Researcher at Broad Institute
Publications - 10
Citations - 1664
Kaijie Zheng is an academic researcher from Broad Institute. The author has contributed to research in topics: CRISPR & Gene. The author has an hindex of 7, co-authored 7 publications receiving 1315 citations. Previous affiliations of Kaijie Zheng include Massachusetts Institute of Technology & McGovern Institute for Brain Research.
Papers
More filters
Journal ArticleDOI
Genome-wide CRISPR screen in a mouse model of tumor growth and metastasis
Sidi Chen,Sidi Chen,Neville E. Sanjana,Kaijie Zheng,Ophir Shalem,Kyungheon Lee,Xi Shi,David A. Scott,Jun S. Song,Jen Q. Pan,Ralph Weissleder,Hakho Lee,Feng Zhang,Phillip A. Sharp +13 more
TL;DR: In this paper, a genome-wide CRISPR/Cas9-mediated loss-of-function screen in tumor growth and metastasis was described. But the authors focused on the effect of mutations on primary tumor growth positively correlates with the development of metastases.
Journal ArticleDOI
Cas13b Is a Type VI-B CRISPR-Associated RNA-Guided RNase Differentially Regulated by Accessory Proteins Csx27 and Csx28
Aaron Smargon,David Benjamin Turitz Cox,Neena Pyzocha,Kaijie Zheng,Kaijie Zheng,Kaijie Zheng,Ian Slaymaker,Ian Slaymaker,Ian Slaymaker,Jonathan S. Gootenberg,Omar Abudayyeh,Patrick Essletzbichler,Patrick Essletzbichler,Patrick Essletzbichler,Sergey Shmakov,Sergey Shmakov,Kira S. Makarova,Eugene V. Koonin,Feng Zhang +18 more
TL;DR: It is established that two class 2 CRISPR-Cas systems that lack Cas1 and Cas2 and encompass a single large effector protein, Cas13b, along with one of two previously uncharacterized associated proteins, can achieve RNA interference when heterologously expressed.
Journal ArticleDOI
High-resolution interrogation of functional elements in the noncoding genome
Neville E. Sanjana,Neville E. Sanjana,Jason Wright,Jason Wright,Kaijie Zheng,Kaijie Zheng,Ophir Shalem,Ophir Shalem,Pierre Fontanillas,Julia Joung,Julia Joung,Christine S. Cheng,Christine S. Cheng,Aviv Regev,Aviv Regev,Feng Zhang,Feng Zhang +16 more
TL;DR: A CRISPR screen using ~18,000 single guide RNAs targeting >700 kilobases surrounding the genes NF1, NF2, and CUL3, which are involved in BRAF inhibitor resistance in melanoma, finds that noncoding locations that modulate drug resistance also harbor predictive hallmarks ofnoncoding function.
Genome-wide CRISPR Screen in a Mouse Model of Tumor Growth and Metastasis
Kyungheon Lee,Jun S. Song,Ralph Weissleder,Hakho Lee,Sidi Chen,Neville E. Sanjana,Kaijie Zheng,Ophir Shalem,Xi Shi,David Scott,Jennifer Q. Pan,Feng Zhang,Phillip A. Sharp +12 more
TL;DR: This study demonstrates Cas9-based screening as a robust method to systematically assay gene phenotypes in cancer evolution in vivo and demonstrates the effect of mutations on primary tumor growth positively correlates with the development of metastases.
Posted ContentDOI
Cas13b is a Type VI-B CRISPR-associated RNA-Guided RNase differentially regulated by accessory proteins Csx27 and Csx28
Aaron Smargon,David Benjamin Turitz Cox,Neena Pyzocha,Kaijie Zheng,Ian Slaymaker,Jonathan S. Gootenberg,Omar Abudayyeh,Patrick Essletzbichler,Sergey Shmakov,Kira S. Makarova,Eugene V. Koonin,Feng Zhang +11 more
TL;DR: In this article, a computational sequence database mining approach was used to identify two Class 2 CRISPR-Cas systems (subtype VI-B) that lack Cas1 and Cas2 and encompass a single large effector protein, Cas13b, along with one of two previously uncharacterized associated proteins, Csx27 or Csax28.