Jurgen Kooren

TL;DR: The data demonstrate that CTCF is directly involved in chromatin architecture and regulates local balance between active and repressive chromatin marks and postulate that throughout the genome, relative position and stability of C TCF-mediated loops determine their effect on enhancer-promoter interactions, with gene insulation as one possible outcome.

TL;DR: The data demonstrate that CTCF is directly involved in chromatin architecture and regulates local balance between active and repressive chromatin marks and postulate that throughout the genome, relative position and stability of C TCF-mediated loops determine their effect on enhancer-promoter interactions, with gene insulation as one possible outcome.

TL;DR: The intricacies of 3C and new 3C-based methods including the 4C, 5C and ChIP-loop assay are discussed, for example, to demonstrate that regulatory DNA elements communicate with distant target genes through direct physical interactions that loop out the intervening chromatin fiber.

TL;DR: The most recent insight into the three-dimensional organization of gene expression is discussed, which shows that regulatory DNA elements communicate with the genes through physical interaction, which loops out the intervening chromatin fiber.

TL;DR: This work used recently established I/11 cells as a model system that faithfully recapitulates the in vivo erythroid differentiation program to study the molecular events that accompany and underlie ACH formation, and used knock-out mice to show that the erythyroid transcription factor p45 NF-E2, which has been implicated in β-globin gene regulation, is dispensable for β- globin A CH formation.