Kaelin D. Fleming

TL;DR: In this paper, the crystal structure of the protein kinase Akt1 has been determined, revealing an autoinhibitory interface between the PH and kinase domains that is often mutated in cancer and overgrowth disorders.

TL;DR: Using a combined biochemical/biophysical approach, insight is revealed into regulation of kinase activity, specifically defining how immunodeficiency and oncogenic mutations of R1021 in the C-terminus can inactivate or activate enzyme activity.

TL;DR: In this paper, the authors report the structure of a heterodimeric PI3Kγ complex, p110γ-p101, which is a unique assembly of catalytic and regulatory subunits.

TL;DR: Detailed biochemical characterisation of Rab-GEF specificity of the human TRAPPII complex is presented, and molecular insight into Rab binding is provided, to provide insight into the GTPase specificity of TRAPPii, and how clinical mutations disrupt this regulation.

TL;DR: In this article, a combination of biochemical assays, hydrogen deuterium exchange mass spectrometry (HDX-MS) and electron microscopy was used to examine the regulation of transport protein particle complexes in solution and on membranes.