Karen L. Reddy

TL;DR: Three-dimensional DNA-immunoFISH is devised for inducible tethering of genes to the inner nuclear membrane (INM) and targeted adenine methylation is used to show that, as is the case for the model system, inactive immunoglobulin loci at the nuclear periphery are contacted by INM and lamina proteins.

TL;DR: It is shown here that VRI and PDP1 proteins feed back and directly regulate dClock expression, comprising a second feedback loop in the Drosophila clock that gives rhythmic expression of dClock, and probably of other genes, to generate accurate circadian rhythms.

TL;DR: Genomic repositioning assays show that LADs, spanning the developmentally regulated IgH and Cyp3a loci contain discrete DNA regions that associate chromatin with the nuclear lamina and repress gene activity in fibroblasts, revealing a mechanism that couples nuclear compartmentalization of chromatin domains with the control of gene activity.

TL;DR: Localization of specific chromatin domains to the nuclear lamina is mediated by YY1 and lamin A/C and is dependent on a specific histone modification profile.

TL;DR: It is shown that the transcription factor EBF restored the generation of CD19+ pro–B cells from Ikaros-deficient hematopoietic progenitors, and is an obligate component of a network that regulates B cell fate commitment and immunoglobulin heavy-chain gene recombination.