K
Karen Sachs
Researcher at Stanford University
Publications - 45
Citations - 6875
Karen Sachs is an academic researcher from Stanford University. The author has contributed to research in topics: Bayesian network & Graphical model. The author has an hindex of 17, co-authored 39 publications receiving 5961 citations. Previous affiliations of Karen Sachs include Massachusetts Institute of Technology & Harvard University.
Papers
More filters
Journal ArticleDOI
Single-Cell Mass Cytometry of Differential Immune and Drug Responses Across a Human Hematopoietic Continuum
Sean C. Bendall,Erin F. Simonds,Peng Qiu,El-ad David Amir,Peter O. Krutzik,Rachel Finck,Robert V. Bruggner,Rachel D. Melamed,Angelica Trejo,Olga Ornatsky,Robert S. Balderas,Sylvia K. Plevritis,Karen Sachs,Dana Pe'er,Scott D. Tanner,Garry P. Nolan +15 more
TL;DR: Single-cell “mass cytometry” analyses provide system-wide views of immune signaling in healthy human hematopoiesis, against which drug action and disease can be compared for mechanistic studies and pharmacologic intervention.
Journal ArticleDOI
Causal Protein-Signaling Networks Derived from Multiparameter Single-Cell Data
Karen Sachs,Karen Sachs,Karen Sachs,Omar D. Perez,Omar D. Perez,Omar D. Perez,Dana Pe'er,Dana Pe'er,Dana Pe'er,Douglas A. Lauffenburger,Douglas A. Lauffenburger,Douglas A. Lauffenburger,Garry P. Nolan,Garry P. Nolan,Garry P. Nolan +14 more
TL;DR: Reconstruction of network models from physiologically relevant primary single cells might be applied to understanding native-state tissue signaling biology, complex drug actions, and dysfunctional signaling in diseased cells.
Journal ArticleDOI
Extracting a cellular hierarchy from high-dimensional cytometry data with SPADE
Peng Qiu,Erin F. Simonds,Sean C. Bendall,Kenneth D. Gibbs,Robert V. Bruggner,Michael D. Linderman,Karen Sachs,Garry P. Nolan,Sylvia K. Plevritis +8 more
TL;DR: This work presents a versatile computational approach, spanning-tree progression analysis of density-normalized events (SPADE), which facilitates the analysis of cellular heterogeneity, the identification of cell types and comparison of functional markers in response to perturbations.
Journal ArticleDOI
Normalization of mass cytometry data with bead standards
Rachel Finck,Erin F. Simonds,Astraea Jager,Smita Krishnaswamy,Karen Sachs,Wendy J. Fantl,Dana Pe'er,Garry P. Nolan,Sean C. Bendall +8 more
TL;DR: The protocol described here includes simultaneous measurements of beads and cells on the mass cytometer, subsequent extraction of the bead‐based signature, and the application of an algorithm enabling correction of both short‐ and long‐term signal fluctuations.
Journal ArticleDOI
Multiplexed mass cytometry profiling of cellular states perturbed by small-molecule regulators
Bernd Bodenmiller,Eli R. Zunder,Rachel Finck,Tiffany J. Chen,Erica S. Savig,Robert V. Bruggner,Erin F. Simonds,Sean C. Bendall,Karen Sachs,Peter O. Krutzik,Garry P. Nolan +10 more
TL;DR: In this article, mass-tag cellular barcoding (MCB) was applied to characterize human peripheral blood mononuclear cell (PBMC) signaling dynamics and cell-to-cell communication, signaling variability between PBMCs from eight human donors, and the effects of 27 inhibitors.