K
Kazuaki Kakehi
Researcher at Kindai University
Publications - 225
Citations - 6786
Kazuaki Kakehi is an academic researcher from Kindai University. The author has contributed to research in topics: Capillary electrophoresis & Glycan. The author has an hindex of 46, co-authored 225 publications receiving 6519 citations. Previous affiliations of Kazuaki Kakehi include Kyowa Hakko Kirin Co., Ltd. & Shimadzu Corp..
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Journal ArticleDOI
High-performance liquid chromatography of reducing carbohydrates as strongly ultraviolet-absorbing and electrochemically sensitive 1-phenyl-3-methyl-5-pyrazolone derivatives
TL;DR: Reverse-phase partition chromatography on a column of Capcell Pak C18 with uv or electrochemical detection allowed rapid analysis of aldoses and N-acetylhexosamines with the detection limit of 1 pmol or 100 fmol, respectively, and proved especially useful for analysis of component monosaccharides of glycorproteins.
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Comparison of the methods for profiling glycoprotein glycans--HUPO Human Disease Glycomics/Proteome Initiative multi-institutional study.
Yoshinao Wada,Parastoo Azadi,Catherine E. Costello,Anne Dell,Raymond A. Dwek,Hildegard Geyer,Rudolf Geyer,Kazuaki Kakehi,Niclas G. Karlsson,Koichi Kato,Nana Kawasaki,Kay-Hooi Khoo,Soo Hyun Kim,Akihiro Kondo,Erika Lattova,Yehia Mechref,Eiji Miyoshi,Kazuyuki Nakamura,Hisashi Narimatsu,Milos V. Novotny,Nicolle H. Packer,Hélène Perreault,Jasna Peter-Katalinić,Gottfried Pohlentz,Vernon N. Reinhold,Pauline M. Rudd,Pauline M. Rudd,Akemi Suzuki,Naoyuki Taniguchi +28 more
TL;DR: The results of this multi-institutional study indicate that MS-based analysis appears as the efficient method for identification and quantitation of oligosaccharides in glycomic studies and endorse the power of MS for glycopeptide characterization with high sensitivity in proteomic programs.
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The protease-activated receptor-2 agonist induces gastric mucus secretion and mucosal cytoprotection
Atsufumi Kawabata,Mitsuhiro Kinoshita,Hiroyuki Nishikawa,Ryotaro Kuroda,Minoru Nishida,Hiromasa Araki,Naoki Arizono,Yasuo Oda,Kazuaki Kakehi +8 more
TL;DR: PAR-2 activation triggers the cytoprotective secretion of gastric mucus by stimulating the release of CGRP and tachykinins from sensory neurons, and the PAR-2-mediated salivary exocrine secretion appears to be independent of capsaicin-sensitive sensory neurons.
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Comparison of Methods for Profiling O-Glycosylation HUMAN PROTEOME ORGANISATION HUMAN DISEASE GLYCOMICS/PROTEOME INITIATIVE MULTI-INSTITUTIONAL STUDY OF IgA1
Yoshinao Wada,Anne Dell,Stuart M. Haslam,Bérangère Tissot,Kevin Canis,Parastoo Azadi,Malin Bäckström,Catherine E. Costello,Gunnar C. Hansson,Yoshiyuki Hiki,Mayumi Ishihara,Hiromi Ito,Kazuaki Kakehi,Niclas G. Karlsson,Catherine A. Hayes,Koichi Kato,Koichi Kato,Nana Kawasaki,Kay-Hooi Khoo,Kunihiko Kobayashi,Daniel Kolarich,Akihiro Kondo,Carlito B. Lebrilla,Miyako Nakano,Hisashi Narimatsu,Jan Novak,Milos V. Novotny,Erina Ohno,Nicolle H. Packer,Elizabeth Palaima,Matthew B. Renfrow,Michiko Tajiri,Kristina A. Thomsson,Hirokazu Yagi,Shin-Yi Yu,Naoyuki Taniguchi +35 more
TL;DR: An assessment of the methodologies currently used for O-glycan analysis found two general strategies were found to give the most reliable data, namely direct MS analysis of mixtures of permethylated reduced glycans in the positive ion mode and analysis of native reduced glycoproteins in the negative ion mode using LC-MS approaches.
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Analysis of the oligosaccharides in ovalbumin by high-performance capillary electrophoresis.
TL;DR: The oligosaccharides in ovalbumin as a glycoprotein model were released with anhydrous hydrazine, and reductively pyridylaminated after re-N-acetylation, and analyzed by capillary zone electrophoresis with on-column fluorometric detection.