Laurence S. Kaminsky

TL;DR: Results provide strong evidence that P450 1A1 catalyzes the hydroxylations of E2 at the C-2, C-15 alpha, and C-6 alpha positions in incubations with microsomes from TCDD-treated MCF-7 cells, but suggest TCDd may also induce a cytochrome P450 E2 4-hydroxylase that is distinct from P450 2A1 or P 450 1A2.

TL;DR: This investigation was undertaken to optimize DNase I treatment of RNA with respect to DNA removal and mRNA preservation and found that incubation of 1 microgram RNA with 1 U of DNase for 30 min at 37 degrees C followed by heat-denaturation of the enzyme for 5 min at 75 degrees C was sufficient to destroy all the contaminating DNA, while completely preserving the respective mRNAs.

TL;DR: The metals in PAH/metal mixtures could diminish PAH carcinogenicity by decreasing induction of their bioactivation by CYP1A1/1A2.

TL;DR: Quercetin, a natural dietary constituent, is a potent inhibitor of estrone sulfatase in vitro, and thus has the potential to express antiestrogenic activity in vivo.

TL;DR: Results suggest that CYP2D6 could be regulated tissue specifically using tissue-specific alternative mRNA splicing, which could regulate the expression of CYP 2D6 protein.