L
Lukasz A. Joachimiak
Researcher at University of Texas Southwestern Medical Center
Publications - 56
Citations - 2457
Lukasz A. Joachimiak is an academic researcher from University of Texas Southwestern Medical Center. The author has contributed to research in topics: Biology & Chemistry. The author has an hindex of 17, co-authored 34 publications receiving 1975 citations. Previous affiliations of Lukasz A. Joachimiak include Fred Hutchinson Cancer Research Center & Howard Hughes Medical Institute.
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Journal ArticleDOI
Computational redesign of protein-protein interaction specificity.
Tanja Kortemme,Lukasz A. Joachimiak,Lukasz A. Joachimiak,Alex N. Bullock,Alex N. Bullock,Aaron D. Schuler,Barry L. Stoddard,David Baker +7 more
TL;DR: A 'computational second-site suppressor' strategy to redesign specificity at a protein-protein interface is developed and applied to create new specifically interacting DNase-inhibitor protein pairs, demonstrating that the designed switch in specificity holds in in vitro binding and functional assays.
Journal ArticleDOI
The Molecular Architecture of the Eukaryotic Chaperonin TRiC/CCT
Alexander Leitner,Lukasz A. Joachimiak,Andreas Bracher,Leonie Mönkemeyer,Thomas Walzthoeni,Thomas Walzthoeni,Bryan Chen,Sebastian Pechmann,Susan Holmes,Yao Cong,Boxue Ma,S. Ludtke,Wah Chiu,F. Ulrich Hartl,Ruedi Aebersold,Ruedi Aebersold,Judith Frydman +16 more
TL;DR: This work integrates chemical crosslinking, mass spectrometry, and combinatorial modeling to reveal the definitive subunit arrangement of TRiC and explains all available crosslink experiments, provides a rationale for previously unexplained structural features, and reveals a surprising asymmetry of charges within the chaperonin folding chamber.
Journal ArticleDOI
The chaperonin TRiC blocks a huntingtin sequence element that promotes the conformational switch to aggregation.
Stephen Tam,Christoph Spiess,Christoph Spiess,William Churk-Nam Auyeung,Lukasz A. Joachimiak,Bryan Chen,Michelle A Poirier,Judith Frydman +7 more
TL;DR: This work determines how the chaperonin TRiC suppresses Htt aggregation and illustrates how molecular chaperones, which recognize hydrophobic determinants, can prevent aggregation of polar polyQ tracts associated with neurodegenerative diseases.
Journal ArticleDOI
Chemical cross-linking/mass spectrometry targeting acidic residues in proteins and protein complexes
Alexander Leitner,Lukasz A. Joachimiak,Pia Unverdorben,Thomas Walzthoeni,Judith Frydman,Friedrich Förster,Ruedi Aebersold +6 more
TL;DR: A chemistry to cross-link acidic residues that generates structural information complementary to that obtained by amine-specific cross-linking, thus significantly expanding the scope of XL-MS analyses and expanding the yield of structural information that can be obtained from cross- linking studies and used in hybrid modeling approaches.
Journal ArticleDOI
Inert and seed-competent tau monomers suggest structural origins of aggregation.
Hilda Mirbaha,Dailu Chen,Olga A. Morozova,Kiersten M. Ruff,Apurwa M Sharma,Xiaohua Liu,Mohammad Goodarzi,Rohit V. Pappu,David W. Colby,Hamid Mirzaei,Lukasz A. Joachimiak,Marc I. Diamond +11 more
TL;DR: It is suggested that initiation of pathological aggregation could begin with conversion of tau monomer from an inert to a seed-competent form.