M. C. Pedroso de Lima

TL;DR: The results indicate that negatively charged ternary complexes of cationic liposomes, DNA and transferrin, or fusigenic peptides, can facilitate efficient transfection of cultured cells, and that they may alleviate the drawbacks of the use of highly positively charged complexes for gene delivery in vivo.

TL;DR: Through in vitro viability tests, the hydrogel-based formulations were shown to be nontoxic and the in vivo injection of a co-cross-linking formulation revealed that the gel was rapidly formed and localized at the injection site, remaining in position for at least 1 week.

TL;DR: In this review considerations are made regarding the structure-activity relationships of cationic liposome/DNA complexes and the key formulation parameters influencing the features of lipoplexes are presented and discussed in terms of their effect on biological activity.

TL;DR: The potential of transferrin (Tf)‐associated liposomes for siRNA complexation and gene silencing is evaluated.

TL;DR: A combination strategy involving cationic liposomes to condense and carry DNA, transferrin to facilitate cellular uptake, and the pH‐sensitive peptide GALA to promote endosome destabilization resulted in significant expression of a luciferase gene in macrophages.