Henrique Faneca

TL;DR: This review focuses on the various parameters that are considered to be crucial to optimize the use of cationic lipid-DNA complexes for gene therapy purposes, from the biophysical aspects underlying the formation of the complexes to the different biological barriers that need to be surpassed for gene expression to occur.

TL;DR: This review will focus on various parameters governing lipoplex biological activity, from their mode of formation to invivo behaviour, in an attempt to dissect the different barriers that need to be surpassed for efficient gene expression to occur.

TL;DR: This review summarizes the different suicide gene systems and gene delivery vectors addressed to cancer, with particular emphasis on recently developed systems and associated bystander effects and provides some insights into the future directions of this approach, particularly towards cancer stem cell eradication.

TL;DR: Through in vitro viability tests, the hydrogel-based formulations were shown to be nontoxic and the in vivo injection of a co-cross-linking formulation revealed that the gel was rapidly formed and localized at the injection site, remaining in position for at least 1 week.

TL;DR: Different cationic lipid-based gene delivery systems consisting of both liposomes and nonliposomal structures, in terms of their in vitro transfection activity, resistance to the presence of serum, protective effect against nuclease degradation and stability under different storage conditions are characterized.