M.R. Sairam

TL;DR: The functional properties of an alternately spliced form of sheep testicular FSH receptor cDNA that codes for a protein similar to a previously described active receptor but differs in the carboxy terminus in sequence and is also shorter by 25 residues are studied.

TL;DR: A sheep testicular cDNA library constructed in pcDNA1 vector was screened with a probe generated by polymerase chain reaction (PCR) and corresponding to a 1.6 kb fragment of the rat luteinizing hormone receptor cDNA to obtain 95% of the putative full-length ovine follicle-stimulating hormone receptor (oFSH-R) cDNA.

TL;DR: It is suggestive that oFSHR-P can substitute for FSH in the development of a contraceptive vaccine as the effects observed are near identical to that seen following immunization with FSH.

TL;DR: Investigation of expression in HEK 293 cells of a cloned cDNA encoding the first eight exons of the FSH receptor along with a carboxyterminal extension that contributed a hypothetical single transmembrane domain reveals for the first time that the seven trans Membrane structure of FSH‐receptor is not absolutely necessary for cell surface expression and hormone binding provided other compensating motifs are present in the protein structure for membrane insertion.

TL;DR: The cloning and characterization of two alternately spliced forms of ovine testicular follitropin receptor mRNA are reported, suggesting that their putative protein products are likely to possess moderate or high affinity binding sites of physiological significance.