Madoka Suzuki

TL;DR: Following application of ultrasounds to cells treated with BTNPs, fluorescence imaging of ion dynamics revealed that the synergic stimulation is able to elicit a significant cellular response in terms of calcium and sodium fluxes; moreover, tests with appropriate blockers demonstrated that voltage-gated membrane channels are activated.

TL;DR: A ratiometric nanothermometer (RNT) for intracellular temperature measurement in real time is developed and successfully followed the temperature change in individual RNT spots in a single cell together with the Ca(2+) burst induced by the Ca (2+) ionophore ionomycin.

TL;DR: It is found that the heat production in a single HeLa cell occurred with some time delay after the ionomycin-induced Ca(2+) influx from the extracellular space, and the increase in temperature was suppressed when Ca( 2+)-ATPases were blocked by thapsigargin.

TL;DR: A small molecule fluorescent thermometer targeting mitochondria (Mito thermo yellow) successfully demonstrates the ability to monitor the intracellular temperature gradient, generated by exogenous heating, in various cells.

TL;DR: The first small molecule fluorescent thermometer selectively targeting the endoplasmic reticulum (ER thermo yellow) is reported, with the highest sensitivity reported so far (3.9%/°C), and successfully demonstrates the ability to monitor intracellular temperature gradients generated by external heat sources in various cell types.