Maeva Le Guennec

TL;DR: U-ExM enables near-native expansion microscopy of samples in vitro and in cells and combined with super-resolution microscopy unveiled details of ultrastructural organization, such as centriolar chirality, that could otherwise be observed only by electron microscopy.

TL;DR: U-ExM as mentioned in this paper is an extension of expansion microscopy that allows the visualization of preserved ultrastructures by optical microscopy, which reveals details of centriole chirality that were previously accessible only by electron microscopy.

TL;DR: Using cryo–electron tomography and subtomogram averaging of centrioles from four evolutionarily distant species, it is found that MTTs are bound together by a helical inner scaffold covering ~70% of the centriole length that maintains M TTs cohesion under compressive forces.

TL;DR: Multifocal flat illumination for field-independent imaging (mfFIFI) enables patterned illumination over an extended field of view that allows for high-speed, multicolor, volumetric super-resolution imaging over 100 × 100 µm 2.

TL;DR: Cryo‐electron tomography of centrioles from four evolutionarily distant species is used to report on the architectural diversity of the centriole's proximal cartwheel‐bearing region, revealing that the cartwheel central hub is constructed from a stack of paired rings with cartwheel inner densities inside.