Susanne Borgers

TL;DR: U-ExM enables near-native expansion microscopy of samples in vitro and in cells and combined with super-resolution microscopy unveiled details of ultrastructural organization, such as centriolar chirality, that could otherwise be observed only by electron microscopy.

TL;DR: Using cryo–electron tomography and subtomogram averaging of centrioles from four evolutionarily distant species, it is found that MTTs are bound together by a helical inner scaffold covering ~70% of the centriole length that maintains M TTs cohesion under compressive forces.

TL;DR: It is established that POB15 is recruited after the cartwheel protein CrSAS-6 and before tubulin glutamylation takes place, and WDR90, the human homolog of POC16, localizes to a region of human centrioles that this work proposes is analogous to the central core of Chlamydomonas Centrioles.

TL;DR: Inherent interaction properties of tubulin provide a structural basis for flagellar microtubule doublet assembly, and the dynamics of B-microtubule nucleation and its distinctive isotropic elongation was elucidated by using live imaging.

TL;DR: It is revealed that POC16 and its human homolog WDR90 are components of the centriolar microtubule wall along the central core region of theCentriole, and it is demonstrated that WDR 90 depletion impairs the localization of inner scaffold components, leading to centriole structural abnormalities in both human and Chlamydomonas cells.